Liquid Culture Systems for in vitro Plant Propagation

372 Barbara Ruffoni et al.
4. Discussion
Both in other bulbous plants and for Ixia, the liquid culture seems to be
particularly promising for the scale-up of mass-production of healthy and
virus-free plants. In fact, the presence of Fusarium oxisporum and Bean
Yellow Mosaic Virus in the propagated material is a serius bottle neck for
the exploitation of the commercial importance of this ornamental plant (De
Hertogh and Le Nard, 1993). The addition of ANC in the cultures seems to
inhibit biomass proliferation but also seems to promote differentiation as
reported also by Chen and Ziv (2001) in Narcissus. In Ixia, the calli
histological analysis and the behaviour of the neoformed propagules indicate
the activation of the embryogenetic pathway in the presence of ANC.
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