Liquid Culture Systems for in vitro Plant Propagation

Tissue culture of Corydalis 561
Figure 2: In vitro propagation of Corydalis yanhusuo via somatic embryogenesis and
recurrent somatic embryogenesis.
(A) Proliferating tuber derived callus on MS basal medium supplemented with 2 mg l -1 BA
and 0.5 mg l -1 NAA.
(B) Somatic embryos showing development of cotyledonary leaves after five weeks of culture
on MS basal medium supplemented with 1.0 mg l -1 zeatin.
(C) Converted somatic embryo with well-developed roots and cotyledonary leaves on half
strength MS basal medium supplemented with 6 % sucrose for one month.
(D) Development of secondary somatic embryos on the surface of converted somatic embryo
after two months of culture on MS basal medium supplemented with ABA.
(E) Synchronized development of secondary somatic embryos on converted primary somatic
embryos on 2.0 mg l -1 ABA containing medium.
(F) Converted secondary somatic embryos after culture in MS liquid medium supplemented
with 0.1 mg l -1 GA 3.
(G) Converted secondary somatic embryos cultured on medium with 5.0 mg l -1 GA 3 showing
about eight shoots and roots.
(H) Converted embryos showing developed tuber on MS medium devoid of phytohormones
after four months of incubation.
(I) Hardened plant derived from the somatic embryo.