Liquid Culture Systems for in vitro Plant Propagation

Tissue culture of Corydalis 563
Table 1: Effect of different concentrations of ABA, paclobutrazol, ancymidoe, GA3, and
PEG-4000 in the medium on the production of of D,L-tetrahydropalmatine and D-corydaline
(mg/g -1 dry weight) by culturing tubers of somatic embryo-derived plants of Corydalis
yanhusuo for one month
Treatment
(mg l -1 )
THP content
(mg l -1 DW � standard error)
COR content
(mg l -1 DW � standard error)
None 1.01 � 0.14 0.53 � 0.22
ABA 0.5 1.33 � 0.55 2.23 � 0.51
1.0 1.44 � 0.35 0.81 � 0.28
2.0 1.45 � 0.15 1.93 � 0.49
5.0 2.30 � 0.98 2.22 � 1.58
Pac 0.5 1.04 � 0.13 1.19 � 0.25
1.0 1.06 � 0.28 1.22 � 0.69
2.0 0.49� 0.16 0.68 � 0.28
5.0 0.86 � 0.17 0.54 � 0.14
10.0 0.50 � 0.10 0.41 � 0.29
Anc 0.5 0.13 � 0.03 0.15 � 0.04
1.0 0.82 � 0.35 1.86 � 0.78
2.0 1.24 � 0.42 1.54 � 0.48
5.0 1.10 � 0.44 1.40 � 0.50
GA3 0.5 0.39 � 0.03 0.19 � 0.03
1.0 1.22 � 0.21 0.24 � 0.10
2.0 1.23 � 0.15 0.69 � 0.17
5.0 0.62 � 0.09 0.32 � 0.15
PEG 15 0.52 � 0.36 0.44 � 0.23
25 1.06 � 0.38 1.28 � 0.33
50 1.04 � 0.29 0.84 � 0.26
100 0.59 � 0.18 0.38 � 0.15
Commercial crude drug 0.35 � 0.08 0.33 � 0.10
With our regeneration system, a synchronized development of embryos
on converted primary somatic embryos, by using appropriate concentration
of ABA, can be achieved. Secondary embryos developed on 2.0 mg l -1 ABA
were uniform in size (Figure 2 E) and the embryos converted within 15 days
of culture in media with different phytohormones. The conversion of
embryos was found to be optimum in the GA3-containing media
(Figure 2 F). Converted somatic embryos cultured on medium with
5.0 mg l –1 GA3 and sucrose 6 % (w/v) developed about eight thin shoots and