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Liquid Culture Systems for in vitro Plant Propagation

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Nutrition of plants <strong>in</strong> greenhouses and <strong>in</strong> <strong>vitro</strong> 487<br />

commercial grower such a high fertilization rate because of the danger of<br />

root decay at such high SSE. We must emphasise that with such high rates of<br />

fertilization of mother plants, careful attention to the water content of the<br />

pots is essential; they must never be allowed to dry out.<br />

Table 8: The effect of alternative RS-media compared with M. et al. (1972) medium on some<br />

floral characters of Sa<strong>in</strong>tpaulia ionantha (from Ayeh, 1998). Registrations were done 3 weeks<br />

after appearance of the first flower, i.e. after 17 (M-72), 16 (RS-1997-1) and 14 weeks (RS-<br />

1997-3)<br />

Mean number per plant<br />

Medium Floral buds Floral stalks Flowers<br />

M- 72 20.2 b 3.3 a 8.3 b<br />

RS-1997-1 24.2 ab 3.0 a 10.0 ab<br />

RS-1997-3 32.5 a 4.9 a 19.3 a<br />

*<br />

Different letters represent statistical differences at 5% level analysed by SAS Statistical<br />

package (ANOVA).<br />

Table 9: The effect of nitrogen fertilization (Ca(NO 3) 2 to the mother plants on percentage of<br />

shoot <strong>for</strong>m<strong>in</strong>g leaf discs and number of shoots per leaf disc (Begonia x cheimantha; Borgen,<br />

1983) as determ<strong>in</strong>ed after 10 weeks of culture<br />

Fertilizer Ca(NO 3) 2<br />

(N=15.5%, Ca = 19.45%)<br />

N<br />

75<br />

Nitrogen and calcium fertilisation <strong>in</strong> mg l -1<br />

Ca<br />

86<br />

N<br />

150<br />

Ca<br />

184<br />

N<br />

300<br />

No. of shoots per leaf disc 48.6 40.0 81.4<br />

% of leaf discs produc<strong>in</strong>g<br />

shoots<br />

55.0 66.0 83.2<br />

4. Uptake of nutrients related to climate<br />

Ca<br />

368<br />

The greenhouse environment consists of many factors that affect both the<br />

uptake of nutrients and their distribution with<strong>in</strong> the plant, thus affect<strong>in</strong>g the<br />

growth of the crop, the yield and the quality of the product.

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