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Liquid Culture Systems for in vitro Plant Propagation

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Development and validation of low cost bioreactor 517<br />

We analysed the FCs content of these different qualities of the biomass<br />

(Figure 9) and it revealed that the less green was the biomass, the lower its<br />

content was (less than 50%).<br />

Fresh weight (g)<br />

250<br />

200<br />

150<br />

100<br />

50<br />

0<br />

0 10 20 30 40<br />

Time (days)<br />

Figure 5: Time course of Ruta shoots growth when cultivated <strong>in</strong> 3-litre bubble flask (Figure<br />

2, Bioreactor 2).<br />

Figure 6: Comparison of Ruta shoot growth cultivated <strong>in</strong> 3-litre bubble flasks (Figure 2,<br />

Bioreactor 2) <strong>in</strong> a medium conta<strong>in</strong><strong>in</strong>g 10 or 30 g l -1 of sucrose.

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