Liquid Culture Systems for in vitro Plant Propagation

Norway spruce somatic embryogenesis 301
exclude the possibility that higher concentration of PEG could interfere with
further plantlet development. On the other hand, our results suggest that low
concentration of PEG can improve Norway spruce somatic embryogenesis
without negative, or with minimal impact, on embling performance.
mature somatic embryos per g FW tissue
90
80
70
60
50
40
30
20
10
0
C111 AFO M22 W2
cell line
0 % PEG
1.87 % PEG
3.75 % PEG
5 % PEG
7.5 % PEG
Figure 4: The effect of PEG on the yield of mature Norway spruce somatic embryos grown
on membrane rafts. Maturation medium was supplemented with 0 – 7.5 % (w/v) PEG 4000.
Bars indicate SE.
% of germination
100
90
80
70
60
50
40
30
20
10
0
0 % PEG
1.87 % PEG
3.75 % PEG
5 % PEG
7.5 % PEG
C111 AFO M22 W2
cell line
Figure 5: The effect of PEG on the germination of desiccated Norway spruce somatic
embryos grown on membrane rafts. Maturation medium was supplemented with 0 – 7.5 %
(w/v) PEG 4000. Bars indicate SE.