Liquid Culture Systems for in vitro Plant Propagation

Development and validation of low cost bioreactor 519
Figure 9: Furanocoumarin levels in the Ruta shoots cultivated up to high density in the 3litre
bubble flask bioreactor (Figure 2, Bioreactor 2). Shoot contents are presented as a
function of their colour: deep-green (external part), yellow-green (medium part) and yellowwhite
(centre of the shoot mass).
FCs concentration in the medium (mg. l -1 )
Realtive FCs level (%)
160
140
120
100
80
60
40
20
0
100
90
80
70
60
50
40
30
20
10
0
deep-green yellow -green yellow -w hite
Biomass aspect
experiment 1 experiment 2
0 1 1 3 10 24 0 1 1 3 10 24 0 1 1 3 10 24 0 1 1 3 10 24
Time (hours)
Pso 8Mop 5,8Mop 5Mop
Figure 10: Furanocoumarin release by Ruta shoots when the culture medium is replaced by a
fresh one (control) containing umbelliferone and or Tween20 as precursor and permeabilizing
agent, respectively. Experiments conducted in 3-litre bubble flasks (Figure 2, Bioreactor 2)
after 20 weeks of growth.