Liquid Culture Systems for in vitro Plant Propagation

Micropropagation of Rosa 381
The rooted microshoots (Figure 3c-d) were incubated in rooting vessel for
different times before their transfer to greenhouse conditions. It was
observed that highest survival percentage (96.7% in R. damascena and 100%
in R. bourboniana) was recorded after 6 weeks of incubation (Table 3 and
4). However, at this stage, most of the shoots were touching the top of the
box (Figure 3e) and therefore, required immediate transplantation. These
plantlets were kept in a greenhouse for 6-8 weeks before their transfer to
larger pots (20cm) (Figure 3f) and eventually, to the fields.
3.3 Comparative response of micropropagation of R. damascena
and R. bourboniana
A comparative analysis of different stages of micropropagation of Rosa
damascena and R. bourboniana indicates that these two species did not have
much difference in respect to different test parameters under the in vitro
culture systems tested (Table 4).
Table 1: Development of cultured shoots of R. damascena in agar-gelled and liquid MS+BAP
(5µmol sucrose (3% (w/v))
Medium
Gelled
(Agar:
0.8 % w/v))
Liquid
No. of shoots
Initial Final*
7.2
± 0.7
7.2
± 0.37
30.4
± 4.1
58.0
± 2.60
* Data recorded after 6 weeks of culture
Shoot length (cm)
Initial Final*
1.08
± 0.03
1.04
± 0.02
1.62
± 0.03
2.96
± 0.2
Stem thickness (mm)
Initial Final*
0.90
± 0.04
0.92
± 0.1
Table 2: Comparison of rooting response* in liquid and agar-gelled media**
1.54
± 0.1
1.96
± 0.05
Liquid medium Agar-gelled medium
Time to root induction 8 days 10 days
Rooting (%) 85.8 5%
Root length (mm) 3.5-4.0 1.5-2.5
Root number per shoot 8-10 3-6
*Data recorded after 3weeks
**1/2 MS+ IBA (10.0 �mol) + sucrose (3% (w/v)) for 1week, then transfer to 1/2 strength
MS without PGRs