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Liquid Culture Systems for in vitro Plant Propagation

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Application of temporary immersion <strong>in</strong> Phalaenopsis 237<br />

Figure 5: Shoot size categories of Phalaenopsis cv. Jaun<strong>in</strong>a after 12-week culture on<br />

0.5 mg l -1 TDZ-conta<strong>in</strong><strong>in</strong>g medium (below) or after 7 weeks on TDZ-medium followed by<br />

5-week culture on TDZ-free medium.<br />

Both size groups are significantly different accord<strong>in</strong>g to Chi-Square Test (p < 0.001).<br />

3.4 Effect of immersion frequency on root<strong>in</strong>g<br />

Shoot elongation dur<strong>in</strong>g the root<strong>in</strong>g phase and the number of roots<br />

produced per shoot can be regulated by aux<strong>in</strong> type and concentration.<br />

Application of 0.5 mg l -1 IAA led to elongation of the shoot axis and leaves<br />

(Figure 6 A), whereas 1.0 mg l -1 NAA <strong>in</strong>hibited shoot elongation<br />

significantly and <strong>in</strong>duced thicker roots (Figure 6 B).

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