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Liquid Culture Systems for in vitro Plant Propagation

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Temporary immersion system: a new concept 183<br />

Increase <strong>in</strong> efficacy<br />

(x fold) compared with<br />

agar<br />

Shoot No. x 7, shoot<br />

length <strong>in</strong>creased, root<strong>in</strong>g<br />

faster and more efficient<br />

Hyperhydricity<br />

Subsequent<br />

successful<br />

acclimatization<br />

Not determ<strong>in</strong>ed Not determ<strong>in</strong>ed<br />

Shoot FW x 4 No Not determ<strong>in</strong>ed<br />

Shoot biomass (FW) x 1 No<br />

Date palm: callus FW x<br />

3.2, better quality<br />

Carrot: callus FW x 1.9,<br />

better quality and plant<br />

regeneration<br />

Yes (shoots and<br />

flowers larger<br />

after)<br />

No Not determ<strong>in</strong>ed<br />

Shoot FW x 1.8 No Not determ<strong>in</strong>ed<br />

Shoot FW x 1.2,<br />

and shoot health x 2<br />

Shoot No. x 2.6,<br />

shoot weight x 2.1,<br />

shoot length x 1.2<br />

Yes but reduced<br />

compared to agar<br />

medium<br />

No with 5 m<strong>in</strong><br />

every 1 h;<br />

Yes with 5 m<strong>in</strong><br />

every 30 m<strong>in</strong><br />

Yes<br />

Not determ<strong>in</strong>ed<br />

Shoot No. x 2.5 No Not determ<strong>in</strong>ed<br />

Authors<br />

Harris and Mason,<br />

1983<br />

Tisserat and<br />

Vandercook, 1985<br />

Tisserat and<br />

Vandercook, 1985<br />

Tisserat and<br />

Vandercook, 1985<br />

Tisserat and<br />

Vandercook, 1985<br />

Aitken-Christie<br />

and Jones, 1987<br />

Krueger et al.,<br />

1991<br />

Alvard et al.,<br />

1993

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