Liquid Culture Systems for in vitro Plant Propagation

350 Anna Mikula et al.
Table 4: Effect of GA3 and kinetin (KIN) on the number of mature somatic embryos formed
by 100 mg of G. cruciata embryogenic cells from suspension culture plated on agar medium
supplemented with 434 µmol AS*. Data were collected between 6 th - 10 th week of the culture
KIN
(µmol)
0.0
2.32
4.64
9.28
Average
SE2=2.98
0.0 1.49 2.89
Average
SE1=3.4
GA3
(µmol)
0.0 1.49 2.89
Average
SE1=3.26
Fraction 240-450 �m a SE3=5.965 Fraction >450 �m b SE3=5.646
257 a
139 ef
231 b
138 ef
189 c
161 d
202 c
129 f
146 de
126 f
150 de
146 de
191 a 170 b 142 c
197 a
142 b
194 a
137 b
103 cde
128 ab
135 a
117 bc
120 a
SE2=2.82
85 f
82 f
105 cd
89 def
87 ef
81 f
83 f
76 f
90 b 82 c
92 b
97 b
107 a
94 b
Table 5: Effect of GA 3 and kinetin (KIN) on the number of mature somatic embryos formed
by 100 mg of G. tibetica embryogenic cells from suspension culture plated on agar medium
supplemented with 434 µmol AS*. Data were collected between 6 th - 10 th week of the culture
KIN
(µmol)
0.0
2.32
4.64
9.28
Average
SE2=2.8
0.0 1.49 2.89
Average
SE1=3.27
GA3
(µmol)
0.0 1.49 2.89
Average
SE1=4.059
Fraction 240-450 �m b SE3=5.659 Fraction >450 �m a SE3=7.030
0 e
0 e
0 e
0 e
201 d
223 bc
291 a
288 a
213 cd
230 b
288 a
290 a
0 b 251 a 255 a
138 c
151 b
193 a
193 a
0 d
0 d
0 d
0 d
0 b
SE2=3.5
510 c
581 b
634 a
620 a
524 c
592 b
629 a
630 a
586 a 594 a
345 c
391 b
421 a
417 a
* Data represent the average of 6 replicates (two independent experiments with 3 Petri plates).
Effect of the aggregate fraction on embryo production was analysed by means of 1-factor
variance analysis, effect of GA 3 and KIN and correlation between them was analysed by 2factor
analysis. SE1 – standard error for kinetin; SE2 – for GA3; SE3 – for aggregate fractions.