Liquid Culture Systems for in vitro Plant Propagation

270 Katerina Grigoriadou et al.
of continuous culture in the TIS for 30 days (Table 2, Figure 6).
Hyperhydricity was apparent but not as serious as in the other liquid system
devices tested. In the case where cultures in TIS were combined with agar
solidified medium (10 and 20 days, respectively) the number of new
microshoots per explant was 0.64 with a significant increased shoot length of
1.40 cm (Table 2). When explants were maintained for 20 days in TIS
followed by 10 days in agar solidified medium, the respective results were
1.41 and 0.70 cm (Table 2). In both treatments hyperhydricity problems
were not observed.
Cultures in agar-solidified medium, which were used as control, resulted
in 1.75 new microshoots per explant of 1.22 cm average shoot length
(Table 2).
a b
c
c d
Figure 4: Culture of olive microshoots in Erlenmeyer flasks under agitation (a and b) and on
filter paper bridges in test tubes (c and d). In d, arrows point to callus masses.