Liquid Culture Systems for in vitro Plant Propagation

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Somatic embryogenesis of Gentiana 349 Table 1: Percentage of aggregate fractions in 3-year-old cell suspension of G. cruciata and G. tibetica (in 1.0 ml of suspension, after 5 days of subculture) Aggregate fraction (�m) G. cruciata G. tibetica 70 –120 54 ± 0.59 36± 0.70 120-240 29 ± 0.54 30 ± 0.67 240-450 11 ± 0.37 14 ± 0.51 450-500 6 ± 0.27 17 ± 0.55 >500 0.6 ± 0.09 3 ± 0.24 Table 2: Ratio (R) of coefficient of fresh (FM) and dry (DM) mass of cell suspensions cultured in MS supplemented with Dic + NAA + BAP + AS Age of culture Gentiana cruciata Gentiana tibetica FM* DM* R FM* DM* R 1-year-old 4.0 3.1 0.78 3.8 3.2 0.84 2-year-old 4.1 3.4 0.83 3.4 3.8 1.12 3-year-old 2.2 2.0 0.91 1.4 2.6 1.86 * ratio of final to initial cell mass Table 3: 2C value describe by cytometic DNA (pg) content in control plant, 3-year-old PEM and regenerants of G. cruciata and G. tibetica. Mitotic activity of PEM - the ratio of cells in phase G 2 to phase G 1 (in bold) Species Control plants PEM Regenerants G. cruciata 2.45 ± 0.13 G. tibetica 2.51 ± 0.036 1.5 ± 0.03 (6%) 1.15 ± 0.35 (18 %) 2.77 ± 0.65 2.74 ± 0.68
350 Anna Mikula et al. Table 4: Effect of GA3 and kinetin (KIN) on the number of mature somatic embryos formed by 100 mg of G. cruciata embryogenic cells from suspension culture plated on agar medium supplemented with 434 µmol AS*. Data were collected between 6 th - 10 th week of the culture KIN (µmol) 0.0 2.32 4.64 9.28 Average SE2=2.98 0.0 1.49 2.89 Average SE1=3.4 GA3 (µmol) 0.0 1.49 2.89 Average SE1=3.26 Fraction 240-450 �m a SE3=5.965 Fraction >450 �m b SE3=5.646 257 a 139 ef 231 b 138 ef 189 c 161 d 202 c 129 f 146 de 126 f 150 de 146 de 191 a 170 b 142 c 197 a 142 b 194 a 137 b 103 cde 128 ab 135 a 117 bc 120 a SE2=2.82 85 f 82 f 105 cd 89 def 87 ef 81 f 83 f 76 f 90 b 82 c 92 b 97 b 107 a 94 b Table 5: Effect of GA 3 and kinetin (KIN) on the number of mature somatic embryos formed by 100 mg of G. tibetica embryogenic cells from suspension culture plated on agar medium supplemented with 434 µmol AS*. Data were collected between 6 th - 10 th week of the culture KIN (µmol) 0.0 2.32 4.64 9.28 Average SE2=2.8 0.0 1.49 2.89 Average SE1=3.27 GA3 (µmol) 0.0 1.49 2.89 Average SE1=4.059 Fraction 240-450 �m b SE3=5.659 Fraction >450 �m a SE3=7.030 0 e 0 e 0 e 0 e 201 d 223 bc 291 a 288 a 213 cd 230 b 288 a 290 a 0 b 251 a 255 a 138 c 151 b 193 a 193 a 0 d 0 d 0 d 0 d 0 b SE2=3.5 510 c 581 b 634 a 620 a 524 c 592 b 629 a 630 a 586 a 594 a 345 c 391 b 421 a 417 a * Data represent the average of 6 replicates (two independent experiments with 3 Petri plates). Effect of the aggregate fraction on embryo production was analysed by means of 1-factor variance analysis, effect of GA 3 and KIN and correlation between them was analysed by 2factor analysis. SE1 – standard error for kinetin; SE2 – for GA3; SE3 – for aggregate fractions.
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LIQUID CULTURE SYSTEMS FOR IN VITRO
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A C.I.P. Catalogue record for this
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Contents Contributing Authors xiii
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Liquid culture systems for in vitro
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Contributing Authors Adelberg, J. 4
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Preface Plant cell, tissue and orga
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Chapter 1 General introduction: a p
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General introduction 3 2. Cell susp
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General introduction 5 rooted in gr
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General introduction 7 (Winkelmann
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General introduction 9 the bioreact
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General introduction 11 (TIS), resu
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General introduction 13 Barz W, Rei
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General introduction 15 Hohe A, Win
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General introduction 17 Shimazu T &
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I. Bioreactors
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22 Wayne R. Curtis 1. Introduction
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24 Wayne R. Curtis headspace double
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26 Wayne R. Curtis that is required
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28 Wayne R. Curtis C L y � P O2
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30 Wayne R. Curtis since there is a
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32 Wayne R. Curtis 5 cm 30 cm 30 o
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34 Wayne R. Curtis Pˆ N � � me
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36 Wayne R. Curtis Radial Flow Impe
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38 Wayne R. Curtis CS = Medium diss
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40 Wayne R. Curtis Murashige T & Sk
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42 Anne Kathrine Hvoslef-Eide et al
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Chapter 4 Practical aspects of bior
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Practical aspects of bioreactor app
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Chapter 5 Simple bioreactors for ma
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Simple bioreactors for mass propaga
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96 K. Y. Paek et al. opportunity fo
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98 K. Y. Paek et al. disadvantages
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100 K. Y. Paek et al. 3.1 Dissolved
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102 K. Y. Paek et al. optimizing bi
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104 K. Y. Paek et al. cosmetics, wh
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106 K. Y. Paek et al. protocol, mor
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108 K. Y. Paek et al. use. In order
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110 K. Y. Paek et al. a b c d e f F
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112 K. Y. Paek et al. a b c d e f F
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114 K. Y. Paek et al. Escalona M, L
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116 K. Y. Paek et al. Son SH & Paek
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118 Seppo Sorvari et al. economical
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120 Seppo Sorvari et al. membrane w
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122 Seppo Sorvari et al. 3. Results
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124 Seppo Sorvari et al. D.O. 160 1
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Chapter 8 Cost-effective mass cloni
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Cost-effective mass cloning of plan
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144 Eun-Joo Hahn & Kee-Yoeup Paek 1
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146 Eun-Joo Hahn & Kee-Yoeup Paek F
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148 Eun-Joo Hahn & Kee-Yoeup Paek T
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150 Eun-Joo Hahn & Kee-Yoeup Paek 3
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152 Eun-Joo Hahn & Kee-Yoeup Paek E
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Chapter 10 Control of growth and di
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Control of growth and differentiati
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Chapter 11 Temporary immersion syst
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Temporary immersion system: a new c
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198 Elio Jiménez González 1. Intr
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200 Elio Jiménez González Several
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202 Elio Jiménez González Figure
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204 Elio Jiménez González exploit
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206 Elio Jiménez González weeks i
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208 Elio Jiménez González germina
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210 Elio Jiménez González Escalan
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Chapter 13 Use of growth retardants
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Use of growth retardants for banana
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Chapter 14 Somatic embryo germinati
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Somatic embryo germination of Psidi
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Somatic embryo germination of Psidi
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232 Tino Hempfling & Walter Preil N
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234 Tino Hempfling & Walter Preil 3
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236 Tino Hempfling & Walter Preil F
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238 Tino Hempfling & Walter Preil F
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240 Tino Hempfling & Walter Preil 4
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242 Tino Hempfling & Walter Preil R
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244 C. Damiano et al. Over the last
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246 C. Damiano et al. 2.2 Temporary
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248 C. Damiano et al. Table 3: Wate
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250 C. Damiano et al. hyperhydricit
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Chapter 17 Optimisation of growing
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Optimisation of growing conditions
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264 Katerina Grigoriadou et al. cul
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266 Katerina Grigoriadou et al. 2.4
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268 Katerina Grigoriadou et al. mic
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270 Katerina Grigoriadou et al. of
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272 Katerina Grigoriadou et al. 4.
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274 Katerina Grigoriadou et al. Tei
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276 Ch. Wawrosch et al. possesses s
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278 Ch. Wawrosch et al. 3. Results
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280 Ch. Wawrosch et al. References
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Chapter 20 Propagation of Norway sp
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Propagation of Norway Spruce 285 2.
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Propagation of Norway Spruce 287 su
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Propagation of Norway Spruce 289 5.
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Propagation of Norway Spruce 291 bl
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Propagation of Norway Spruce 293 H
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296 M. Vágner et al. 1995). In liq
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Mass propagation of conifer trees 4
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Chapter 31 Potentials for cost redu
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Potentials for cost reduction 405 c
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Potentials for cost reduction 407 c
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Potentials for cost reduction 409 s
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Potentials for cost reduction 411 N
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Potentials for cost reduction 413 G
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Chapter 32 A new approach for autom
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A new approach for automation 417 (
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A new approach for automation 419 p
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A new approach for automation 421 T
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A new approach for automation 423 D
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426 B. McAlister et al. 1. Introduc
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428 B. McAlister et al. 2.2 Multipl
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430 B. McAlister et al. Table 2: Mu
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432 B. McAlister et al. rest time -
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434 B. McAlister et al. Figure 3: M
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436 B. McAlister et al. Average mul
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438 B. McAlister et al. Table 4: Co
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440 B. McAlister et al. Semi-solid
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442 B. McAlister et al. Escalona M,
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444 Jeffrey Adelberg including Host
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446 Jeffrey Adelberg BioSystems (Ho
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448 Jeffrey Adelberg more prolific
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450 Jeffrey Adelberg vessel of liqu
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452 Jeffrey Adelberg Figure 4: Labo
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454 Jeffrey Adelberg constituted a
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456 Jeffrey Adelberg Acknowledgemen
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Chapter 35 Aeration stress in plant
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Aeration stress in plant tissue cul
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476 Hans R. Gislerød et al. 1. Int
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478 Hans R. Gislerød et al. biorea
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480 Hans R. Gislerød et al. A low
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482 Hans R. Gislerød et al. Table
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484 Hans R. Gislerød et al. common
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486 Hans R. Gislerød et al. can be
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488 Hans R. Gislerød et al. 4.1 Li
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490 Hans R. Gislerød et al. 4.3 Ca
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492 Hans R. Gislerød et al. Morten
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494 Han Bouman & Annemiek Tiekstra
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V. Biomass for Secondary Metabolite
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510 E. Gontier et al. 1. Introducti
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512 E. Gontier et al. 2. Materials
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514 E. Gontier et al. developed dra
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516 E. Gontier et al. 3.3 Establish
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518 E. Gontier et al. Fresh weight
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520 E. Gontier et al. 3.5 Effect of
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522 E. Gontier et al. 3.6 Scale up
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524 E. Gontier et al. Lorenzo JC, G
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526 Dirk Wilken et al. 1. Introduct
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528 Dirk Wilken et al. running at 6
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530 Dirk Wilken et al. described a
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532 Dirk Wilken et al. packed cell
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534 Dirk Wilken et al. bioactive co
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536 Dirk Wilken et al. Fowler MW (1
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Chapter 40 Cultivation of root cult
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Cultivation of root cultures of Pan
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Chapter 41 Optimisation of Panax gi
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Optimisation of Panax ginseng liqui
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558 S. M. Nalawade et al. performan
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560 S. M. Nalawade et al. Plantlets
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562 S. M. Nalawade et al. leaves on
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564 S. M. Nalawade et al. roots (Fi
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Chapter 43 Production of taxanes in
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Production of taxanes 569 2. Materi
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Production of taxanes 571 Figure 2:
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Production of taxanes 573 various T
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Acknowledgments The editors thank t
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578 Contents 130, 131, 133, 134, 13
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580 Contents poplar, see Populus Po
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582 Contents bubble aerated 165 bub
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584 Contents ginsenoside content, p
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586 Contents plant growth regulator
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588 Contents -free microcorms 71 -f
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