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OP-SM01 Sports Medicine 1<br />

or the influence <strong>of</strong> age or gender apart from one study (McFarlin et al. 2006) that showed a lower TLR4 expression in active compared<br />

with sedentary subjects in both a young and an elderly population.<br />

The purpose <strong>of</strong> this study was to examine the effects <strong>of</strong> the volume <strong>of</strong> self-reported moderate-strenuous exercise training on human<br />

monocyte TLR4 expression in a large cohort <strong>of</strong> active subjects (minimum <strong>of</strong> 3 hours moderate-strenuous training per week). With local<br />

ethics committee approval 85 healthy subjects aged 18-53 yrs, body mass 52-112 kg; BMI 18-35 kg/m2 completed a self-report questionnaire<br />

<strong>of</strong> weekly exercise training load and a resting venous blood sample (following an overnight fast) was taken for analysis <strong>of</strong> circulating<br />

monocyte counts and TLR4 expression (corrected for non-specific binding using an isotype control) as described by Lancaster et al.<br />

(2005). TLR4 expression was compared in males and females matched for habitual activity level, young and older subjects matched for<br />

habitual activity level and subjects classed according to hours <strong>of</strong> training per week (3-7 , 8-14 and 15-25 h/week). T-test and ANOVA were<br />

used to analyse the data.<br />

Monocyte counts were in the normal range for all subjects and were not influenced by age, gender or habitual activity level. Monocyte<br />

TLR4 expression (geometric mean fluorescence intensity, GMFI, mean [SEM]) was not affected by age (GMFI: 20.4 [1.5] in 47 subjects aged<br />

18-24 yrs versus 23.7 [2.3] in 23 subjects aged 30-53 yrs, P=0.240) or gender (GMFI: 19.7 [1.2] in 52 men versus 23.7 [2.3] in 31 women,<br />

P=0.096). There was no significant main effect for physical activity level for TLR4 expression (F = 0.805, P=0.451) which was 22.2 [2.1], 21.8<br />

[1.6] and 18.6 [1.3] (GMFI) in subjects exercising for 3-7, 8-14 and 15-25 h/week, respectively.<br />

This study showed that TLR4 expression is not affected by exercise training load, gender or age in a physically active population.<br />

References<br />

Gleeson M, et al. (2006). Exerc Immunol Rev 12: 34-53.<br />

Lancaster GI, et al. (2005). J Physiol 563(3): 945-955.<br />

McFarlin BK, et al. (2006). J Gerontol 61(4): 388-393.<br />

VALIDATION OF INFRARED THERMOGRAPHY AS INJURY PREVENTION METHOD IN PROFESSIONAL SOCCER PLAYERS.<br />

GÓMEZ, P., NOYA, J., NUÑEZ, J., FERNANDEZ, I., SILLERO, M.<br />

1. FACULTY OF PHYSICAL ACTIVITY AND SPORT SCIENCES-INEF. UNIVERSIDAD POLITÉCNICA DE MADRID (UPM), 2. VF SPORT, 3. REAL<br />

ZARAGOZA CF, SAD<br />

Introduction.<br />

Tensiomiography (TMG) is been used as an injury prevention method in pr<strong>of</strong>essional soccer players. It records the muscle response to a<br />

known electrical stimulus intensity [1,2]. In other hand, Thermography (TG) is a technique used for detecting and managing injuries [3], in<br />

which a camera records the infrared radiation released from the metabolic activity <strong>of</strong> human body for estimating temperatures on different<br />

areas [4]. This study attempts to correlate both techniques in order to validate the TG as an injury prevention method in pr<strong>of</strong>essional<br />

footballers.<br />

Methods: Using a Tensiomyographer (TMG 100 Measurement System, TGM - BMC Ltd.) and a Thermographic camera (ThermaCAM TM<br />

SC640, FLIR SYSTEMS), 20 pr<strong>of</strong>essional Spanish soccer players were assessed the day after the weekly competition. They were recorded 5<br />

variables by TMG (Delay Time [DT], Contraction Time [CT], Sustain Time [ST], Relaxation Time [RT] y Maximal Displacement [MxD]) in both<br />

Femoral Biceps (dominant [DFB] and non-dominant [NDFB]). Previously, it had been obtained the mean temperatures (T) <strong>of</strong> those areas by<br />

TG.<br />

Results: Student T-test showed lack <strong>of</strong> significant differences between DFB and NDFB for all recorded variables (DTDBF = 26.7, DTNDBF =<br />

27.5; CTDBF = 32.2, CTNDBF = 34.3; STDBF = 191.5, STNDBF = 199.8; RTDBF = 56.5, RTNDBF = 56.0; MxDDBF = 5.8, MxDNDBF = 5.7; TDBF =<br />

33.8, TNDBF = 33.9). Indirect significant correlations were found between T and CT (r = -0.35; p < 0.05) and DT (r = -0.39; p < 0.05) considering<br />

together the results <strong>of</strong> both Femoral Biceps and, dividing the data in DFB and NDFB, they were found indirect significant differences<br />

between T and STDBF (r = -0.50; p < 0.05) and between T and CTNDBF (r = -0.45; p < 0.05) and DTNDBF (r = -0.49; p < 0.05). Considering<br />

individual cases, TG reach similar results compared with TMG on the injury risk for each player.<br />

Discussion and Conclusion.<br />

Differences on the significant variables for the dominant leg may be due to different contraction pattern or function during the game or to<br />

different injuries prevalence for each leg. However, TG may be considered as a valid method in order to assess the injury risk <strong>of</strong> a pr<strong>of</strong>essional<br />

soccer player.<br />

References.<br />

1. D. Križaj, et al. (2008) Short-term repeatability <strong>of</strong> parameters extracted from radial displacement <strong>of</strong> muscle belly. Journal <strong>of</strong> Electromyography<br />

and Kinesiology,18 (4) pp 645-651.<br />

2. http://www.tensiomyography.com/our-star-users/tmg-references.html<br />

3. Garagiola, U. & Giani, E. (1990). Use <strong>of</strong> telethermography in the management <strong>of</strong> <strong>sport</strong>s injuries. Sports Medicine. 10(4): 267-272.<br />

4. Barnes, R.B. (1967). Determination <strong>of</strong> body temperature by infrared emission. J. Appl. Physiol. 22:1143-1146.<br />

INFLUENCE OF COLD WATER FACE IMMERSION ON POST-EXERCISE PARASYMPATHETIC REACTIVATION<br />

AL HADDAD, H., AHMAIDI, S., BUCHHEIT, M.<br />

RESEARCH LABORATORY, EA-3300 «LABORATORY OF EXERCISE PHYSIOLOGY AND REHABILITATION», FACULTY OF SPORT SCIENCES, UNIVER-<br />

SITY OF PICARDIE, JULES VERNE, 80025, AMIENS, FRANCE.<br />

Introduction: A delayed parasympathetic reactivation after exercise is associated with an increased risk <strong>of</strong> sudden cardiac death. Thus,<br />

means <strong>of</strong> improving post-exercise parasympathetic reactivation are now receiving greater interest. Hayashi et al. (1997) showed that cold<br />

water face immersion (CWFI) can increase vagal activity (i.e., inferred from heart rate (HR) variability (HRV)) at rest, but whether this beneficial<br />

effect is preserved during recovery after exercise is not known. Therefore, the aim <strong>of</strong> the present study was to investigate the effect <strong>of</strong><br />

CWFI on parasympathetic reactivation following exercise.<br />

Methods: Eleven men (21.6±1.3 yr; 1.80±0.06 m; 76.1±13.0 Kg) performed, on two different occasions, an intermittent exercise (i.e., a<br />

cycling 30-s Wingate test (to reduce vagal activity) followed by a 5-min run at 60% <strong>of</strong> maximal aerobic velocity, interspersed with 5-min <strong>of</strong><br />

seated recovery). Immediately after the 5-min run, participants underwent a seated passive recovery with either CWFI or without (C) condition.<br />

Participants breathed through a snorkel in both recovery conditions. In CWFI condition, water temperature was kept at 10-12°C.<br />

Parasympathetic reactivation was assessed through beat-to-beat HR collection (Polar Electro, Kempele, Finland) during the 5-min following<br />

the submaximal exercise (Buchheit et al., 2007). Absolute HR recovery was calculated from the immediate 60s period after exercise<br />

116 14 TH<br />

ANNUAL CONGRESS OF THE EUROPEAN COLLEGE OF SPORT SCIENCE

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