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Thursday, June 25th, 2009<br />

CONCLUSIONS OF HUNGARIAN ELITE ATHLETES’ DOPING CONTROLS IN THE YEAR OF THE OLYMPIC GAMES IN BEI-<br />

JING.<br />

TROMBITÁSNÉ RÁNKY, M., NOE, J., FRENKL, R.<br />

SEMMELWIES UNIVERSITY, UNIVERSITY OF PANNONIA VESZPRÉM<br />

Introduction: The <strong>sport</strong>smen have been used different medicines and dietary supplements during the trainings and competitions. Theirs<br />

quantity and quality are disputable by point <strong>of</strong> view <strong>of</strong> health protection many times. According to our opinion it is necessary systematic<br />

health education and scientific nutrition advices to fend <strong>of</strong>f pressure <strong>of</strong> consuming supplements induced by the commercial advertisements<br />

and by psychological reasons. Sport background <strong>of</strong> these acting we should like help on with special data bases. In former researches<br />

we fixed information during long time (7 years) one kind <strong>of</strong> <strong>sport</strong> (athletic). In the present case, our aim gain data about medicine<br />

and supplement consumption habit and specification <strong>of</strong> Hungarian elite athletes <strong>of</strong> different discipline <strong>of</strong> <strong>sport</strong> during one Olympic<br />

year.<br />

Methods: The analyzed information based on the admissions <strong>of</strong> Doping Control Collection Form <strong>of</strong> different Hungarian elite athletes in<br />

Hungary in time from January 01 2008 to December 31 2008. The Forms are recording the names <strong>of</strong> medicines and nutrition supplements<br />

consumed by the competitors ten days prior the control. We have created a database out <strong>of</strong> these data.<br />

Results: The members <strong>of</strong> examination were representatives <strong>of</strong> 32 Olympic-, 12 Paralympics- and 5 non Olympic -disciplines.<br />

The total sum <strong>of</strong> Doping controls was 1518 (n= 851m, n=667w) out during in the given period <strong>of</strong> time.<br />

The competitors <strong>of</strong> Olympic disciplines have been participated 1396 in case n=766m (503 urine samples, 263 blood samples), n=630w<br />

(422 urine s, 208 blood s).<br />

Non Olympic <strong>sport</strong>smen were checking 61 in case, n=43m (41 urine s, 2 blood s), n=18w (17 urine s, 1 blood s).<br />

Paralympics had controls 61 in case, n=42m, n=19w all in urine.<br />

In the period <strong>of</strong> survey only in case 70 (4.6%) hasn’t use any medicines and dietary supplements.<br />

Use <strong>of</strong> the prohibited medications with license were detected 97 (6.38%) in case. (n=50m, n=47w)<br />

Numbers <strong>of</strong> articles named by Hungarian elite athletes were investigated grouped to medicines and nutrition supplements by the athlete<br />

gender and disciplines <strong>of</strong> <strong>sport</strong>s.<br />

Conclusions: It has been discovered from Doping Collecting Forms too many <strong>sport</strong>s men use different non steroid medicines and pain<br />

relieves with together.<br />

After detailed analyzing <strong>of</strong> consumption <strong>of</strong> vitamins, the results showed too many competitors used by more types <strong>of</strong> vitamin complex.<br />

In our opinion there is a high rate <strong>of</strong> consumption if somebody used five or more dietary supplements in the checking period. We was<br />

detected 66 in case (n=49m and n=17w) when the number <strong>of</strong> taken supplements are over then 10.<br />

Conclusions <strong>of</strong> our examination that significant numbers <strong>of</strong> <strong>sport</strong>smen do not used the supplements according to needs.<br />

For optimization <strong>of</strong> nutrition advices should be a theoretically accept for <strong>sport</strong>s, like the standards RDA, ODI, PDI.<br />

References:<br />

D H Catlin, K D Fitch, A Ljungqvist. (2008) Journal <strong>of</strong> Internal Medicine, 264; 99-114.<br />

Ránky M. (2007) Annual Congress <strong>of</strong> the ECSS abstracts book, 502.<br />

14:15 - 15:15<br />

Poster presentations<br />

PP-MB01 Molecular Biology 1<br />

PHYSICAL ACTIVITY AND EXERCISE-INDUCED LYMPHOCYTE APOPTOSIS<br />

ENSENYAT, A., SAGARRA, L., PORTERO, M., PAMPLONA, R.<br />

UNIVERSITY OF LLEIDA<br />

Introduction: A decrease in lymphocyte counts and changes in its composition has been described after prolonged and sustained vigorous<br />

exercise. To evaluate the contribution <strong>of</strong> apoptosis to lymphopenia during early recovery from acute physical exercise in humans, a<br />

systematic review has been carried out.<br />

Methods: From January 1998 to August 2008, 11 studies investigating the incidence <strong>of</strong> apoptosis in human blood lymphocytes during<br />

early recovery from an acute exercise bout have been published. They comprise <strong>of</strong> 17 effects from a total sample <strong>of</strong> 90 volunteers. Exercise<br />

mode differed greatly among studies and included; short duration (70%VO2max) intensity exercise; prolonged (>120’) exercise; eccentric exercise and progressive maximal exercise. In all cases,<br />

venous blood samples were collected before and early after the exercise bout. In some studies, a third sample time-point was allocated<br />

at some time between 1 hour to 24h <strong>of</strong> recovery.<br />

Lymphocyte apoptosis was analyzed by cytometric procedures or by optic microscopy determination <strong>of</strong> apoptotic morphological<br />

changes.<br />

Results and Discussion: Results show that an intensity threshold can exist as exertion below 60%VO2max did not induce significant<br />

changes in apoptosis parameters such as annexin-V or CD95 expression, or caspasses activities in lymphocytes. On the other hand,<br />

increases in percentages <strong>of</strong> lymphocytes expressing annexin-V or CD95, or in caspasse 8 and 9 activity (Mooren, 2002; Tuan, 2008;<br />

Wang, 2005) have been reported immediately after intense exercise. Sixty minutes after exertion values had returned to resting levels,<br />

except for the percentage <strong>of</strong> NK cells expressing CD95 that were still elevated (Simpson, 2007), and for caspasse 3 activity that increased<br />

at 24 hours <strong>of</strong> recovery (Wang, 2005).<br />

A duration threshold can also exist seeing that percentages <strong>of</strong> annexin-V or CD95 lymphocytes were superior after long duration running;<br />

and persisted elevated for a longer time; than after short duration running at a similar speed (Mooren, 2004; Steensberg, 2002).<br />

Navalta (2005) notes an increase in percentage <strong>of</strong> lymphocytes with morphological signs <strong>of</strong> apoptosis immediately after a progressive<br />

and maximal exercise test that returned to resting levels in the first hour <strong>of</strong> recovery.<br />

OSLO/NORWAY, JUNE 24-27, 2009 189

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