european college of sport science

PP-MB01 Molecular Biology 1
Physical fitness can also regulate apoptosis, as 180’ after a marathon run, the percentage of lymphocytes expressing annexin-V was
significantly elevated from pre-exercise values in bad-trained athletes while in well-trained athletes there was no increase (Mooren,
2004). The percentage of lymphocytes expressing CD95 increased but did not differ among groups.
References
Mooren FC 2002 J Appl Physiol 93(1), 147-53
Mooren FC 2004 Med Sci Sports Exerc 36(9), 1476-83
Navalta JW 2007 Int J Sports Med 28(6), 539-42
Simpson RJ 2007 Res Sports Med 15(3), 157-74
Steensberg A 2002 Eur J Appl Physiol 87(1), 38-42
Tuan R 2008 BJSM 42(1), 11-16
Wang JS 2005 Eur J Appl Physiol 95(4), 290-7
PGC1A GENE VARIATION AND PHYSICAL PERFORMANCE IN LITHUANIAN ATHLETES
GINEVICIENE, V., KUCINSKAS, V.
VILNIUS UNIVERSITY
INTRODUKTION Peroxisome proliferator-activated receptor gamma coactivator-1a (PGC1A) is a transcriptional coactivator with crucial
impact on multiple aspects of cellular energy metabolism including mitochondrial biogenesis and cellular respiration, the regulation of
adaptive thermogenesis, adipocyte cell development and also lipid and glucose metabolism. The most common coding single nucleotide
polymorphism - G1444A (rs8192678) is located in exon eight and cause amino acid substitution Gly482Ser. PGC1A [A] allele is associated
with traits of the metabolic syndrome.
METHOD We tested the hypothesis that the frequency of the minor [A] allele at the PGC1A locus is lower in Lithuanian professional athletes
(cases) [N=551; mean age: 17.7±5.3 yr], than in controls [N=97; mean age: 31.3±13.5 yr]. Athletes represented 4 functional groups: endurance
(N=64); mixed sports (N=33); strength and speed (N=47), and team sports (N=407). Their physical characteristics were (mean ± SD):
height 176±10.4 cm, weight 68.8±15.6 kg, body mass index 21.9±3.4 kg/m2, body muscle mass 36.7±9.5 kg and body fat mass 8±3.2
kg, muscle/fat mass index 5±1.6. All subjects gave informed consent before participating and went through a medical examination.
Genomic DNA was extracted from peripheral blood leukocytes. Genotyping for PGC1A [G/A] polymorphism was performed by PCR and
restriction enzyme digestion. All the statistical analyses were carried out by use of the SPSS program version 13. Allele frequencies were
compared between athletes and controls.
RESULT Genotypes of athletes were identified as [G/G] 52.1%, [G/A] 42.1% and [A/A] 5.8% (χ2=2.83, p=0.09) and the genotypes in
the population samples were [G/G] 42.2%, [G/A] 37.8% and [A/A] 20.0% (χ2=3.79, p=0.055). The frequency of the minor [A] allele
was significantly lower in athletes than in controls (26.9% vs. 38.9%; P = 0.01). In the mixed sports group the frequency of the [A] allele
was less common than in other sports groups: in the endurance group and the strength and speed group the frequency of the [A] allele
was 26.6%, and in the team sports group the frequency of this allele was 27.4%.
DISCUSSION PGC1A [A/A] genotype is less frequent in all Lithuanian athlete groups in comparison to the general population (5.8% vs.
20.0%; P=0.005). The results of the present study imply that the PGC1A [G] allele of the [G/G] genotype is more common in the professional
athlete group than in the general population of Lithuania. In conclusion, the present findings suggest that there is an association
between PGC1A G1444A polymorphim and physical performance in Lithuanian athletes.
HIGH MOLECULAR MASS PROTEOMICAL EVALUATION OF CARDIAC MUSCLE OF RATS SUBMITTED TO AEROBIC EX-
ERCISE.
ROCHA, L., PETRIZ, B., BORGES, D., OLIVEIRA, R., ANDRADE, R., PEREIRA, R., FRANCO, O.
UNIVERSIDADE CATOLICA DE BRASILIA
Several reports describe regular exercise as an important factor for health improvement. This practice could be directly related to con
tractile force development in cardiac cells. In order to evaluate the relations between exercise intensity protein and mRNA expression, 30
isogenics adult male rats Wistar (Rattus norvergicus) were randomly divided into four groups and further submitted to swimming training
during twelve weeks with 2.5% (TG1); 5.0% (TG2) and 7.5% (TG3) overload and one control group (CG) by using sedentary rats. Firstly, our
findings demonstrated clear morphologic alterations, suggesting that the intrinsic contractile characteristics of myofibrils could be modified
in order to contribute for an increase of left ventricle activity. Furthermore, reliable differences at high molecular mass 80 to 240(kDa)
protein maps were observed, by using one and two dimensional electrophoresis .Major proteins were further indentified by MALDI ToF-
ToF. Among these proteins, all treatments demonstrated an increased expression of protein and mRNA of myosin heavy chain in comparison
to CG. Moreover the improvement of other proteins were also observed as troponin, sarcomeric proteins, proteins associated to
oxidative metabolism as oxidoredutases, translocases of mitochondrial membrane and proteins analogous to super
oxide desmutases. These modifications in protein expression patterns suggest a clear correlation to standard intensity obtained in each
training group. One of these effects demonstrated in high intensity when compared to low and moderate, showed to be more efficient in
the MHC enhancing expression in cardiac contractile cells. These alterations seem to demonstrate that exercises of moderate and high
intensity can induce significant molecular and structural alterations in the cardiac contractile cells
EFFECTS OF DIFFERENT INTENSITY AND DURATION OF ENDURANCE TRAINING ON SATELLITE CELL CONTENT IN RAT
SKELETAL MUSCLE
KUROSAKA, M., NAITO, H., ICHINOSEKI-SEKINE, N., OGURA, Y., KAKIGI, R., KATAMOTO, S.
JUNTENDO UNIVERSITY
Introduction: Skeletal muscle satellite cells play an important role for muscle fiber regeneration and hypertrophy. It is well known that
resistance training can increase the number of satellite cells with muscle hypertrophy. Recently, it has been reported that relatively high
intensity endurance training causes proliferation of satellite cells in human skeletal muscle with hypertrophy. Also, we clarified that relative
low intensity and long duration endurance training by using voluntary wheel running induced the number of satellite cells in rat
plantaris muscle without hypertrophy. Therefore, not only exercise intensity but also duration might affect for an increase in satellite cell
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ANNUAL CONGRESS OF THE EUROPEAN COLLEGE OF SPORT SCIENCE