european college of sport science

Friday, June 26th, 2009
EFFECTS OF ENDURANCE AND RESISTANCE TRAINING ON CALCITONIN GENE-RELATED PEPTIDE (CGRP) CONTENT IN
SLOW AND FAST RAT’S MUSCLES
PARNOW, A., GHARAKHANLOU, R., HEDAYATI, M., MAHDIAN, R., RAJABI, S.
UNIVERSITY
Calcitonin Gene-Related Peptide (CGRP), a 37-amino acid peptide generated by alternative processing of primary transcripts from calcitonin
gene, is broadly distributed in the peripheral and central nervous systems of vertebrate and invertebrate species. CGRP plays a
main role in Neuromuscular Junction (NMJ). This paper investigates the effects of Endurance and Resistance training on the content of
CGRP in Slow and Fast Muscles. A number of twenty-two male Wistar rats (10 mo of age, 220± 20 gr, Iran Pasteur Institute) randomly
were divided to three groups (Control (n=7), Endurance training (n=7), and Resistance training (n=8)) and followed 12 weeks training
according protocols. Animals of the resistance training group were housed in metal cage with a wire-mesh tower; Endurance training (5
days a week, 60 min/day, 30 m/min speed) was performed by animals in endurance group. Forty-eight hours after last session
of protocols, animals were anaesthetized with a mixture of KetamineTM and Xylazine. The right soleus (slow muscle) and anterior tibialis
(fast muscle) were removed under sterile condition via an incision on dorsolatral aspect of the hindlimb. After removal, tissues were
frizzed quickly in liquid nitrogen and kept at -70 ° C for later using. For CGRP assay, ELISA kit was used. For data analyses, One-way
ANOVA was used. Data analysis showed that there was not a significant different between control and endurance training groups in
slow and fast muscle’s CGRP. But, the content of CGRP in both fast and slow muscles was significantly different in resistance training
group compared with control group. It is expressed that training can be a main factor for CGRP releasing in muscles. In addition, the type
and intensity of activity probably are contributed to CGRP content increase. Therefore, as recognized role of CGRP in NMJ and former
researches reported that CGRP contant increse in motoneurons followed by exercise, the present research findings that has investeagated
effect of different training on CGRP content in muscles is attractive.
OSLO/NORWAY, JUNE 24-27, 2009 497