european college of sport science

PP-PH07 Physiology 7
enzymes concentration (CK، CK-MB and LDH). For statistical analysis of data, one-way repeated measures and LSD post hoc were
used. No significant differences were found in the increase of serum enzymes concentration (CK، CK-MB and LDH) between three
groups. However, there were significant increase in serum enzymes concentration immediately after and 30 min after exercise in the
three groups. It wasn’t observed a significant difference among the three programs in lactate concentration. Although, the ability of
keeping the repetition (training volume) by using 90s and 120s rest intervals has been more than that of 60s, but statistically it wasn’t
observed a significant difference in training volume. The results of the present study showed that 60, 90, and 120 sec rest duration between
resistance training sets don’t affect on markers of muscle damage such as CK، CK-MB and LDH and also don’t affect on
training volume.
EFFECTS OF CONCURRENT AND RESISTANCE TRAINING ON CALCITONIN GENE-RELATED PEPTIDE (CGRP) CONTENT IN
SLOW AND FAST MUSCLES OF WISTAR RATS
PARNOW, A., GHARAKHANLOU, R., HEDAYATI, M., MAHDIAN, R., GORGIN, Z.
UNIVERSITY
Calcitonin Gene-Related Peptide (CGRP), a 37-amino acid peptide generated by alternative processing of primary transcripts from calcitonin
gene, is broadly distributed in the peripheral and central nervous systems of vertebrate and invertebrate species. The purpose of
the present study was to investigation the effects of Concurrent (Resistance and Endurance) and Resistance Training on the content of
CGRP in Slow and Fast Muscles of Wistar Rats. A number of twenty -three male Wistar rats (10 mo of age, 220 ± 15 gr, Iran Pasteur Institute)
randomly were divided to three groups (control (n=7), concurrent training (n=8), and resistance training (n=8)) and followed 12 weeks
training according protocols. Animals of the resistance group were housed in metal cage with a wire-mesh tower, with two water bottles
set at the top. Concurrent group did a combine of both resistance and endurance training (5 days a week, 60 min/day, 30 m/min speed).
Forty-eight hours after last session of protocols, animals were anaesthetized with a mixture of KetamineTM and Xylazine. The right soleus
(as slow muscle) and anterior tibialis (AT, as fast muscle) were removed under sterile condition via an incision on dorsolatral aspect of the
hindlimb. After remove, tissues were quickly frizzed in liquid nitrogen and kept at -70 ° C for later useage. For CGRP assay, ELISA kit was
used. For data analyses, One-way ANOVA was used. Data analysis showed that there was a significant difference between control and
concurrent training groups in slow muscle CGRP. Also, the content of CGRP in both fast and slow muscles was significantly difference in
resistance training group with control group. Both resistance and concurrent training increased the content of CGRP in the fast and slow
muscles. Therefore, CGRP increase depends on the nature of activity and probably duration and intensity of it, but not the type of muscle.
Finally, CGRP content is depending on activity and stimulus more than muscle type so that concurrent training has caused CGRP increase
in both slow and fast muscles.
EFFECTS OF STRENGTH TRAINING ON CALCITONIN GENE-RELATED PEPTIDE (CGRP) CONTENT IN SLOW AND FAST
MUSCLES OF WISTAR RATS
GHARAKHANLOU, R., ESLAMI, R., PARNOW, A.H., VAHIDPOUR, SH., MADIAN, R.
UNIVERSITY
Calcitonin Gene-Related Peptide (CGRP), a 37-amino acid peptide, generated by alternative processing of primary transcripts from calcitonin
gene, is broadly distributed in the peripheral and central nervous systems of vertebrate and invertebrate species. The purpose of
the present study was to investigate the effect of Strength Training on the content of CGRP in Slow and Fast Muscles of Wistar Rats. A
number of 12 male Wistar rats (10 mo of age, 220 ± 15 gr) randomly were divided to tow groups (control (n=7) and strength training (n=5)).
The strength training protocol consisted of climbing a 1- meter–long ladder set at an 85° angle, with a weight attached to a tail. For CGRP
assay, ELISA kit was used. Data showed that there was not a significant difference between slow and fast muscle CGRP of control group.
Nonetheless, the strength training leaded to increase in CGRP content of slow muscle and decrease in CGRP content of fast muscle that
both increase and decrease were not significant compare with control group (respectively, p= 0.155 , p=0.083). In conclusion, the results
of current study show that slow and fast muscle CGRP content changes with strength training which the quantity and the direction of this
changes may depend on duration and intensity of training protocol.
EFFECTS OF 8 WEEKS OF RESISTANCE TRAINING ON HAEMATOLOGICAL VARIABLES IN MEN
HASSANLOEI, H., AHMADIZAD, S., IBRAHIM, K., ASLANKHANI, M.A.
AALBORG UNIVERSITY, DENMARK & SHAHID BEHESHTI UNIVERSITY G.C., TEHRAN, IRAN.
Resistance exercise or weight training is used extensively to improve physical fitness, enhance performance, prevent injuries, increase
muscle size and also is used in rehabilitation programmes (1). The acute effects of resistance exercise on selected haematological variables
have been recently investigated (2,3). However, no information is available regarding the chronic effects of resistance exercise
haematological parameters. Therefore, the present investigation was designed to examine the effects of 8 weeks of resistance training
on red blood cell and platelet indices. Training group (N = 9; age, 22 ± 1.5 y) undertook a strength-training programme three days a
week for 8 weeks, while the control group (N = 6; age, 23 ± 1 y) were asked to avoid any regular physical activity during this period.
Resistance exercise protocol included the performance of 3 sets of 8-10 repetitions of six exercises at 80% of 1RM. After 4 weeks of training
subjects performed another 1RM test and the exercise intensity was set at 80% of new 1RM for the remaining 4 weeks. Four resting
blood samples were taken before training, after 4 and 8 weeks of training and after 4 days of recovery and were analysed for RBC count,
haemoglobin, haematocrit, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin
concentration (MCHC), red blood cell distribution width (RDW), fibrinogen, platelet count, plateletcrit, mean platelet volume (MPV), and
platelet distribution width (PDW). No significant difference between pre and post-training values was observed in the control group for all
measured platelet variables. Resistance training resulted in a significant increase in muscular strength by 20% to 35%. Statistical analyses
of the data indicated that RBC count, haemoglobin, and haematocrit decreased significantly during first 4 weeks of training and increased
significantly (P < 0.01) between weeks 5 to 8 to nearly pre-training level. However, the other RBC indices did not change significantly
in response to resistance training. A significant effect of training was found for platelet count and plateletcrit (P < 0.01). Post-hoc
analyses revealed a significant increase in platelet count and plateletcrit during first 4 weeks of training with no significant changes
between weeks 5 to 8. However, MPV, PDW, and fibrinogen did not change significantly in response to resistance training. It was concluded
that resistance training doesn’t change red blood cell parameters, while increases platelet count and PCT. Therefore, it could be
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ANNUAL CONGRESS OF THE EUROPEAN COLLEGE OF SPORT SCIENCE