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Introduction to the Agilent 7100 Capillary Electrophoresis System

Introduction to the Agilent 7100 Capillary Electrophoresis System

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Using <strong>the</strong> <strong>Agilent</strong> <strong>7100</strong> <strong>Capillary</strong> <strong>Electrophoresis</strong> <strong>System</strong> 5<br />

<strong>Capillary</strong> <strong>Electrophoresis</strong>, CE<br />

Using an Injection Program<br />

The injection table is used for advanced injection tasks such as:<br />

• Injection from different vials, for example ITP.<br />

• Multiple injection modes, for example spiking.<br />

• Injecting a buffer plug after <strong>the</strong> sample <strong>to</strong> prevent sample loss after<br />

applying voltage.<br />

The online help provides more information on this.<br />

Timetable<br />

In <strong>the</strong> CE Timetable, present on <strong>the</strong> right-hand side of <strong>the</strong> CE Method Setup dialog<br />

you can choose <strong>to</strong> set and change values for <strong>the</strong> following parameters at a<br />

certain time during <strong>the</strong> run:<br />

• Voltage<br />

• Current<br />

• Power<br />

• Pressure<br />

• Inlet vial<br />

• Outlet vial<br />

• Cassette temperature<br />

• Lower alarm limit for current<br />

• High pressure (only in CEC mode)<br />

Time programming of <strong>the</strong>se parameters can be useful for example <strong>to</strong>:<br />

• Define gradients (for voltage or temperature).<br />

• Change <strong>the</strong> buffer during <strong>the</strong> analysis (inlet and outlet).<br />

• Apply pressure during <strong>the</strong> analysis.<br />

• Change <strong>the</strong> outlet vial during analysis.<br />

• Change <strong>the</strong> alarm limit for current for example, when changing <strong>the</strong> buffer<br />

system during <strong>the</strong> analysis.<br />

<strong>Agilent</strong> <strong>7100</strong> <strong>Capillary</strong> <strong>Electrophoresis</strong> <strong>System</strong> User Manual 105

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