Introduction to the Agilent 7100 Capillary Electrophoresis System

5 Using the Agilent 7100 Capillary Electrophoresis System
Capillary Electrophoresis, CE
Capillary Electrophoresis, CE
How to Use Your Agilent CE Instrument for Capillary
Electrophoresis Separations
This chapter introduces method parameters for applications running in CE
mode. This mode is the most suitable for applications in the field of capillary
zone electrophoresis (CZE), micellar electrokinetic chromatography (MEKC)
and capillary isoelectric focussing (CIEF). The current CE mode for your
instrument can be selected under Instrument < more CE < Instrument
configuration... . The current mode is visible in the second line of the CE control
diagram. For detailed information, see “Working with the CE Diagram” on
page 84 and “Graphical User Interface Explanation” on page 86.
Agilent CE Method Parameters
This section describes the parameters of an Agilent CE method for data
acquisition and their meanings. Example sets of parameters for a method to
analyze the test sample are in “Setting Up a Test Sample Run” on page 252.
After you have completely set up the Agilent CE method and supplied the
sample information, you can run your method. Choose Run Method from the
Run control menu.
Overview
A convenient way to access the parameters of an Agilent CE method is the
item Setup Instrument Method in the Instrument menu. This displays the CE
Method Setup dialog box. Tabs with parameters for the DAD and CE part of a
method can be edited on this screen. The left-hand side shows the most
important parameters. The right-hand side offers several pages that can be
expanded subsequently for editing.
92 Agilent 7100 Capillary Electrophoresis System User Manual