Introduction to the Agilent 7100 Capillary Electrophoresis System

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5 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrochromatography, CEC Capillary Electrochromatography, CEC Capillary electrochromatography (CEC) is a fusion of capillary electrophoresis and capillary chromatography. In CEC, solvent is mobilized through a packed capillary column by electroosmotic flow instead of the hydraulic flow used in liquid chromatography LC. Higher efficiencies are achievable because of the uniform flow velocity profile and the ability to use smaller particle sizes in longer columns than is possible with LC. Operation of CEC requires an equal application of high pressure to the inlet and outlet vials in order to suppress bubble formation while avoiding hydrodynamic flow due to a pressure differential. How to Use Your Agilent CE Instrument for Capillary Electrochromatography Requirements • External pressure supply (< 15 bar) of oil-free air or nitrogen. • Push-fit connector and gas line (supplied in startup kit). • Outlet lift connection to the tubing system NOTE For external pressure supply, a male adapter fitting the instrument’s female adapter and PTFE tubing (1/8" OD x 1/16" ID PTFE tubing) are part in the accessory kit of the instrument. Please add a suitable connector to the external pressure supply. The inlet for the external high pressure gas is situated at the back of the instrument. 148 Agilent 7100 Capillary Electrophoresis System User Manual
Using the Agilent 7100 Capillary Electrophoresis System 5 Capillary Electrochromatography, CEC In connector Out connector Liquid cooling External pressure connector Drainage Figure 43 External high pressure gas inlet Agilent 7100 Capillary Electrophoresis System User Manual 149
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Agilent 7100 Capillary Electrophore
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In This Guide… This guide contain
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Contents 1 Introduction 13 Introduc
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Contents Delivery Checklist 55 Inst
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Contents Running the CEC Analysis 1
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Contents 9 Parts and Materials for
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Introduction 1 Where to Find Inform
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Site Requirements and Specification
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Installing the Instrument 3 Unpacki
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Installing the Instrument 3 Install
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Installing the Instrument 3 Install
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LAN Configuration 4 TCP/IP Paramete
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LAN Configuration 4 Initialization
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LAN Configuration 4 Initialization
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LAN Configuration 4 Link Configurat
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LAN Configuration 4 Manual Configur
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LAN Configuration 4 Manual Configur
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LAN Configuration 4 Agilent Bootp S
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Controlling the Agilent CE Instrume
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Using the Agilent 7100 Capillary El
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Maintenance 8 Cleaning the Electrod
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Maintenance 8 Maintenance of the Re
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Maintenance 8 Changing the Air Inle
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Maintenance 8 Changing the Lamp Rem
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Maintenance 8 Changing the Lamp Ins
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Appendix 10 General Safety Informat
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Appendix 10 Allgemeine Sicherheitsi
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Appendix 10 Allgemeine Sicherheitsi
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Appendix 10 Informations générale
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Appendix 10 Informazioni generali s
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Appendix 10 Informazioni generali s
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Appendix 10 Información de segurid
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Appendix 10 安全に関する
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Appendix 10 安全に関する
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Appendix 10 一般安全信息
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Appendix 10 Setting Up a Test Sampl
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Appendix 10 Setting Up a Test Sampl
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Appendix 10 The Waste Electrical an
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Appendix 10 Sound Emission Sound Em
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Appendix 10 Agilent Technologies on
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Index A accessing 199 accessories 5
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www.agilent.com In This Book This m
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