Introduction to the Agilent 7100 Capillary Electrophoresis System

Using the Agilent 7100 Capillary Electrophoresis System 5
Capillary Electrophoresis, Plus High Pressure CE+p
Operation and Function in CE+p Mode
By selecting CE+p mode, some additional functions are available for control
via the CE diagram, which will assist in the operation of capillary gel
electrophoresis (CGE) with highly viscous buffers. This includes the ability to
apply high pressure during preconditioning and postconditioning and the
ability to use high pressure to introduce gel or samples to the capillary.
Preconditioning and Postconditioning
When the instrument is operating in CE+p mode, the user can chose to use the
High Pressure Flush command menu during preconditioning or postconditioning
(Figure 41). This means that high pressure will be applied to the capillary inlet
vial which may be:
• A set value (2-12 bar)
• Raw external pressure (up to 15 bar): Allows pressurizing the inlet vial
and/or the outlet vial. The external pressure up to 15 bar can be applied
directly without reduction.
Agilent 7100 Capillary Electrophoresis System User Manual 143