Introduction to the Agilent 7100 Capillary Electrophoresis System

8 Maintenance
Maintenance of the Replenishment System
Clean Tubes
The Clean Tubes operation is meant to replace liquid in tubing from the
electrolyte bottle to the replenishment needle and from the replenishment
needle to the waste bottle. A vial is lifted to the replenishment needle and
filled several times. The number of filling/emptying cycles can be set by users
using the Lab Advisor/Instrument Utilities software. This operation is meant
for buffer change purposes or for cleaning with water.
It is important to make sure there is enough buffer in the electrolyte bottle for
the Clean Tubes number of filling/emptying cycles specified (for example a
minimum of 50 mL). An excessive Clean Tubes time will drain the electrolyte
bottle, causing air bubbles to be introduced into the system.
The volume in the tubing system from electrolyte bottle and the waste bottle is
approximately 5 mL. Please note that a relatively high amount of buffer is
located in the glass frit of the electrolyte bottle. It is recommended that you
use the Flush Tubes command first to flush the direct tubing connection from
the electrolyte bottle to the waste bottle with at least for example 10-times the
volume of fresh buffer. Please note the individual viscosity of your buffer
system.
NOTE
If the replenishment system will not be used for some time, it should be cleaned first with
water and then with isopropanol by the Flush Tubes and Clean Tubes operations.
Clean Level Sensor
The purpose of the Clean Level Sensor functionality is to dissolve small buffer
crystals which may have formed in the tip of the level sensing needle (short
needle of the double needle) in the replenishment needle assembly. Remaining
pathways of the internal tubing of the level sensing circuit are also cleaned.
One indication to use this function is failed level sensing during operation. The
other indication is a failed IRP (Injection Replenishment and Pressure) level
sensing test from the injection replenishment system tests of the Lab Advisor.
You may use this procedure as well if you leave the replenishment systen
unused for a while.
This function prompts you to load tray position 48 (or user input) with a vial
filled with an appropriate cleaning liquid (usually water or current buffer
system). When initiated, 8 cleaning cycles are performed.
220 Agilent 7100 Capillary Electrophoresis System User Manual