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Introduction to the Agilent 7100 Capillary Electrophoresis System

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Using <strong>the</strong> <strong>Agilent</strong> <strong>7100</strong> <strong>Capillary</strong> <strong>Electrophoresis</strong> <strong>System</strong> 5<br />

<strong>Capillary</strong> Electrochroma<strong>to</strong>graphy, CEC<br />

CEC-Specific GUI Explanation<br />

When <strong>the</strong> CEC mode is selected, a new icon in <strong>the</strong> form of a gas bottle appears<br />

on <strong>the</strong> left right side of <strong>the</strong> CE diagram next <strong>to</strong> <strong>the</strong> tray (see Figure 46 on<br />

page 152).<br />

There are also a number of new parameters which may be set ei<strong>the</strong>r from <strong>the</strong><br />

Edit Entire Method option or from <strong>the</strong> Individual Parameter Settings within <strong>the</strong><br />

Instrument menu selected from <strong>the</strong> Method & Run Control <strong>to</strong>olbar. The new<br />

parameter choices appear in <strong>the</strong> following menu selections.<br />

CEC Method Setup<br />

When CEC mode is selected, a new section for pressure settings appears on <strong>the</strong><br />

left side of <strong>the</strong> Method Setup dialog box. (see Figure 47 on page 154). The<br />

parameter set here is <strong>the</strong> pressure applied <strong>to</strong> both vials in <strong>the</strong> inlet and outlet<br />

lift during <strong>the</strong> electrophoretic run.<br />

Options that can be selected include:<br />

• Pressure off<br />

• Internal, pressure or vacuum up <strong>to</strong> 100 mbar can be applied <strong>to</strong> <strong>the</strong> inlet lift<br />

• Raw External Pressure<br />

• External, set pressure from 2-12 bar<br />

<strong>Agilent</strong> <strong>7100</strong> <strong>Capillary</strong> <strong>Electrophoresis</strong> <strong>System</strong> User Manual 153

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