Introduction to the Agilent 7100 Capillary Electrophoresis System

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5 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Switching the Lamp On The lamp can be switched on automatically as part of initialization (see “DAD Control” on page 180). To switch the lamp on or off manually: 1 In the DAD diagram click the lamp icon. A menu will offer the option to switch the lamp on or off depending of the current status. Please see Figure 23 for the different lamps statuses in the DAD diagram. Off Ignition On Figure 23 UV lamp status Off, Ignition, On 2 To ignite the lamp from the menu go to Instrument > More DAD > UV Lamp and select Switch Lamp On or Switch Lamp Off. Please see Figure 23 for the different lamps statuses in the DAD diagram. 3 Prior to a run, allow the lamp to warm up for about 1 hour. If the warm up time of the lamp is too short, a baseline drift in the electropherogram may occur. 110 Agilent 7100 Capillary Electrophoresis System User Manual
Using the Agilent 7100 Capillary Electrophoresis System 5 Capillary Electrophoresis, CE Preparing the Replenishment System if Needed During multiple analyses, the composition of the buffer can change. One way to refresh the buffer is replenishment. The replenishment system automatically empties and refills the buffer vials in the tray with new buffer from the electrolyte bottle. Typically, replenishment is done before every or after every 3 to 4 runs, depending on the stability and volume of the running buffer. If you do NOT want to use the replenishment system, you still have to put empty bottles (electrolyte and waste) into the replenishment system at the bottom of the instrument. The bottles are also used as a pressure and vacuum reservoir. Missing bottles lead to pressure and/or vacuum error messages. WARNING System under pressure Pressure is applied to the bottles. • Only use bottles with part number 9300-1748 or 5042-6478 with this instrument. These bottles have a protective coating. If you want to use the replenishment system, you have to fill the electrolyte bottle with buffer. Use about 100 mL to make sure the frit is submerged in buffer. Do not fill more than 400 mL buffer into the bottles to ensure the system can work properly. When using buffers containing detergent (for example SDS), the waste bottle should contain some liquid (for example a few mL of isopropanol) to destroy the detergent bubbles. Detailed information on buffers is given in the primer High Performance Capillary Electrophoresis (5990-3777EN), delivered together with the instrument or numerous application notes available on our web page. Make sure the waste bottle is empty so it can hold the same amount of liquid as in the electrolyte bottle. Agilent 7100 Capillary Electrophoresis System User Manual 111
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Agilent 7100 Capillary Electrophore
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In This Guide… This guide contain
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Contents 1 Introduction 13 Introduc
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Contents Delivery Checklist 55 Inst
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Contents Running the CEC Analysis 1
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Contents 9 Parts and Materials for
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Introduction 1 Where to Find Inform
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Site Requirements and Specification
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Installing the Instrument 3 Unpacki
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Installing the Instrument 3 Install
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Troubleshooting and Diagnostics 6 C
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Troubleshooting and Diagnostics 6 A
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Hardware Information 7 7100 Capilla
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Hardware Information 7 Firmware Fir
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Hardware Information 7 Diode Array
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Hardware Information 7 Diode Array
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Maintenance 8 Overview of Maintenan
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Maintenance 8 Overview of Maintenan
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Maintenance 8 Early Maintenance Fee
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Maintenance 8 Cleaning the Electrod
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Maintenance 8 Maintenance of the Re
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Maintenance 8 Changing the Air Inle
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Maintenance 8 Changing the Lamp Rem
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Maintenance 8 Changing the Lamp Ins
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Appendix 10 General Safety Informat
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Appendix 10 Allgemeine Sicherheitsi
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Appendix 10 Allgemeine Sicherheitsi
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Appendix 10 Informations générale
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Appendix 10 Informazioni generali s
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Appendix 10 Informazioni generali s
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Appendix 10 Información de segurid
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Appendix 10 安全に関する
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Appendix 10 安全に関する
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Appendix 10 一般安全信息
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Appendix 10 Setting Up a Test Sampl
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Appendix 10 The Waste Electrical an
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Appendix 10 Sound Emission Sound Em
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Appendix 10 Agilent Technologies on
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Index A accessing 199 accessories 5
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www.agilent.com In This Book This m
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Introduction to the Agilent 7100 Capillary Electrophoresis System

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