Introduction to the Agilent 7100 Capillary Electrophoresis System

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5 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE CAUTION Instrument damage by corrosion Components of the replenishment system can be damaged by aggressive or corrosive buffers, organic solvents or evaporated components from these. • Avoid using aggressive or corrosive buffers or organic solvents that can damage the liquid handling system. Buffers for replenishment or evaporated components from these buffers will come into contact with the following material: polytetrafluoroethylene (PTFE), stainless steel (SST, Grade 316), fluorine rubber (FPM, Viton), polyaryletheretherketone (PEEK), perfluoro-ethylene-propylene (FEP), glass (filter + bottles). A typically acceptable pH range is pH 3-11; for example formic acid can be used in concentrations up to 0.1 %. • If you would like to use a buffer composition exceeding the given conditions, we recommend running an analysis without using the replenishment system but individual vials. Before Filling the Replenishment System The replenishment system (bottles and tubing) should be cleaned when: • Using it for the first time. • Changing the replenishment buffer. • If the replenishment system will be idle for some time. Filling the Replenishment System To prepare the replenishment system. 1 Click one of the replenishment bottles in the CE diagram. 2 Choose Change Bottles to release the pressure before you open the bottles. 3 Open the front door at the bottom of the front panel. WARNING Hazardous organic solvents and sample residues The waste may contain organic solvents and residue from your sample. • Wear rubber gloves and safety glasses when handling electrolytes and deposit the waste in a safe waste container. 112 Agilent 7100 Capillary Electrophoresis System User Manual
Using the Agilent 7100 Capillary Electrophoresis System 5 Capillary Electrophoresis, CE 4 Take out the electrolyte bottle and unscrew the cap. The electrolyte bottle has a frit and tubing inside. Avoid contaminating the frit (see Figure 24 on page 113). Filling the Replenishment Bottles Cap of electrolyte bottle Waste bottle Frit Electrolyte bottle Figure 24 Filling the replenishment bottles Agilent 7100 Capillary Electrophoresis System User Manual 113
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Agilent 7100 Capillary Electrophore
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In This Guide… This guide contain
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Contents 1 Introduction 13 Introduc
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Contents Delivery Checklist 55 Inst
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Contents Running the CEC Analysis 1
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Contents 9 Parts and Materials for
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Introduction 1 Where to Find Inform
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Site Requirements and Specification
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Installing the Instrument 3 Unpacki
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Installing the Instrument 3 Install
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Installing the Instrument 3 Install
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Troubleshooting and Diagnostics 6 C
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Troubleshooting and Diagnostics 6 A
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Hardware Information 7 7100 Capilla
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Hardware Information 7 7100 Capilla
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Hardware Information 7 Firmware Fir
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Hardware Information 7 Diode Array
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Hardware Information 7 Diode Array
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Hardware Information 7 Installing D
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Hardware Information 7 External Wat
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Hardware Information 7 Analog to Di
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Maintenance 8 Overview of Maintenan
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Maintenance 8 Overview of Maintenan
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Maintenance 8 Early Maintenance Fee
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Maintenance 8 Cleaning the Electrod
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Maintenance 8 Maintenance of the Re
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Maintenance 8 Changing the Air Inle
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Maintenance 8 Changing the Lamp Rem
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Maintenance 8 Changing the Lamp Ins
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Appendix 10 General Safety Informat
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Appendix 10 Allgemeine Sicherheitsi
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Appendix 10 Allgemeine Sicherheitsi
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Appendix 10 Informations générale
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Appendix 10 Informazioni generali s
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Appendix 10 Informazioni generali s
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Appendix 10 Información de segurid
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Appendix 10 安全に関する
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Appendix 10 安全に関する
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Appendix 10 一般安全信息
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Appendix 10 Setting Up a Test Sampl
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Appendix 10 Setting Up a Test Sampl
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Appendix 10 The Waste Electrical an
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Appendix 10 Sound Emission Sound Em
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Appendix 10 Agilent Technologies on
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Index A accessing 199 accessories 5
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www.agilent.com In This Book This m
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Introduction to the Agilent 7100 Capillary Electrophoresis System

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