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Introduction to the Agilent 7100 Capillary Electrophoresis System

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Contents<br />

<strong>Agilent</strong> CE Method Parameters 92<br />

Vials 96<br />

Cassette Temperature 97<br />

High Voltage <strong>System</strong> 98<br />

Posttime and S<strong>to</strong>ptime 100<br />

Replenishment 100<br />

Preconditioning 101<br />

Injection 103<br />

Timetable 105<br />

Detec<strong>to</strong>r Parameters 107<br />

Switching <strong>the</strong> Lamp On 110<br />

Preparing <strong>the</strong> Replenishment <strong>System</strong> if Needed 111<br />

Preparing Vials for Buffer and Sample 115<br />

Installing a <strong>Capillary</strong> in <strong>the</strong> Alignment Interface 124<br />

Installing a <strong>Capillary</strong> in an Empty <strong>Capillary</strong> Cassette 128<br />

Inserting <strong>the</strong> Cassette 132<br />

Fraction Collection 135<br />

<strong>Capillary</strong> <strong>Electrophoresis</strong>, Plus High Pressure CE+p 136<br />

Requirements 136<br />

Outlet Lift Connection <strong>to</strong> <strong>the</strong> Tubing <strong>System</strong> 138<br />

Preparing <strong>the</strong> <strong>Agilent</strong> CE Instrument for High Pressure Use 140<br />

Operation and Function in CE+p Mode 143<br />

Injection Options with CE+p 146<br />

Timetable Options with CE+p 146<br />

CE+p Control Using <strong>the</strong> CE Diagram 147<br />

<strong>Capillary</strong> Electrochroma<strong>to</strong>graphy, CEC 148<br />

How <strong>to</strong> Use Your <strong>Agilent</strong> CE Instrument for <strong>Capillary</strong><br />

Electrochroma<strong>to</strong>graphy 148<br />

Preparing <strong>the</strong> <strong>Agilent</strong> CE Instrument for High Pressure Use 150<br />

CEC-Specific GUI Explanation 153<br />

Injection Options with CEC 156<br />

Timetable Options with CEC 157<br />

CEC Control Using <strong>the</strong> CE Diagram 158<br />

8 <strong>Agilent</strong> <strong>7100</strong> <strong>Capillary</strong> <strong>Electrophoresis</strong> <strong>System</strong> User Manual

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