Introduction to the Agilent 7100 Capillary Electrophoresis System

8 Maintenance
Maintenance of the Replenishment System
The Replenishment System Will Not Be Used for Some Time
The replenishment system should be flushed first with water and then with
isopropanol.
Please follow these steps.
1 Open the Lab Advisor or Instrument Utilities software and start monitoring
the respective instrument.
2 In the tools section, select the CE Control screen and click on Change Bottles to
release the pressure before you open the bottles.
3 Take out the electrolyte bottle and unscrew the cap. The electrolyte bottle
has a frit and tubing inside. The frit must not get dirty.
4 Fill a minimum 300 mL of water into the electrolyte bottle. Make sure the
water is filtered by using a 0.25 µm or 0.45 µm filter depending on its
viscosity.
5 Make sure the waste bottle is empty.
6 Cap the bottles tightly.
7 Choose Done in the Change Bottles dialog box. Wait until the pressure has
built up again.
8 To flush the internal tubing system from the electrolyte bottle to the waste
bottle, including all valves on this pathway, select Flush Tubes. During this
step the replenishment needle itself is not flushed. Set a flushing time of 2
minutes.
9 To clean the internal tubing system, including the replenishment, select
Clean Tubes. During this step the replenishment is used to fill and empty a
vial several times. Set the number of repetitions to 5.
10 When requested to do so, place a capped and empty vial into a tray position.
11 Execute the clean level sensing operation to clean the replenishment needle
entirely.
12 Repeat all steps starting with number 2 and use isopropanol instead of
water. Empty the waste bottle whenever you fill or refill the electrolyte
bottle.
218 Agilent 7100 Capillary Electrophoresis System User Manual