Introduction to the Agilent 7100 Capillary Electrophoresis System
Introduction to the Agilent 7100 Capillary Electrophoresis System
Introduction to the Agilent 7100 Capillary Electrophoresis System
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5 Using <strong>the</strong> <strong>Agilent</strong> <strong>7100</strong> <strong>Capillary</strong> <strong>Electrophoresis</strong> <strong>System</strong><br />
<strong>Capillary</strong> Electrochroma<strong>to</strong>graphy, CEC<br />
<strong>Capillary</strong> Electrochroma<strong>to</strong>graphy, CEC<br />
<strong>Capillary</strong> electrochroma<strong>to</strong>graphy (CEC) is a fusion of capillary electrophoresis<br />
and capillary chroma<strong>to</strong>graphy. In CEC, solvent is mobilized through a packed<br />
capillary column by electroosmotic flow instead of <strong>the</strong> hydraulic flow used in<br />
liquid chroma<strong>to</strong>graphy LC. Higher efficiencies are achievable because of <strong>the</strong><br />
uniform flow velocity profile and <strong>the</strong> ability <strong>to</strong> use smaller particle sizes in<br />
longer columns than is possible with LC. Operation of CEC requires an equal<br />
application of high pressure <strong>to</strong> <strong>the</strong> inlet and outlet vials in order <strong>to</strong> suppress<br />
bubble formation while avoiding hydrodynamic flow due <strong>to</strong> a pressure<br />
differential.<br />
How <strong>to</strong> Use Your <strong>Agilent</strong> CE Instrument for <strong>Capillary</strong><br />
Electrochroma<strong>to</strong>graphy<br />
Requirements<br />
• External pressure supply (< 15 bar) of oil-free air or nitrogen.<br />
• Push-fit connec<strong>to</strong>r and gas line (supplied in startup kit).<br />
• Outlet lift connection <strong>to</strong> <strong>the</strong> tubing system<br />
NOTE<br />
For external pressure supply, a male adapter fitting <strong>the</strong> instrument’s female adapter and<br />
PTFE tubing (1/8" OD x 1/16" ID PTFE tubing) are part in <strong>the</strong> accessory kit of <strong>the</strong><br />
instrument. Please add a suitable connec<strong>to</strong>r <strong>to</strong> <strong>the</strong> external pressure supply.<br />
The inlet for <strong>the</strong> external high pressure gas is situated at <strong>the</strong> back of <strong>the</strong><br />
instrument.<br />
148 <strong>Agilent</strong> <strong>7100</strong> <strong>Capillary</strong> <strong>Electrophoresis</strong> <strong>System</strong> User Manual
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