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Introduction to the Agilent 7100 Capillary Electrophoresis System

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5 Using <strong>the</strong> <strong>Agilent</strong> <strong>7100</strong> <strong>Capillary</strong> <strong>Electrophoresis</strong> <strong>System</strong><br />

<strong>Capillary</strong> <strong>Electrophoresis</strong>, Plus High Pressure CE+p<br />

<strong>Capillary</strong> <strong>Electrophoresis</strong>, Plus High Pressure CE+p<br />

The <strong>Agilent</strong> CE instrument is capable of accepting an external gas pressure in<br />

addition <strong>to</strong> <strong>the</strong> internal pressure. The external high pressure source can be up<br />

<strong>to</strong> 15 bar (218 psi), and is regulated within <strong>the</strong> instrument via method settings<br />

or with direct instructions from <strong>the</strong> CE diagram between 2-12 bar. This<br />

pressure can be applied <strong>to</strong> one or both vials at <strong>the</strong> capillary ends. The high<br />

pressure option may be required if using highly viscous replaceable gels in <strong>the</strong><br />

CGE.<br />

Requirements<br />

• External pressure supply (< 15 bar) of oil-free air or nitrogen.<br />

• Push-fit connec<strong>to</strong>r and gas line (supplied in startup kit).<br />

• Outlet lift connection <strong>to</strong> <strong>the</strong> tubing system<br />

NOTE<br />

For external pressure supply, a male adapter fitting <strong>the</strong> instrument’s female adapter and<br />

PTFE tubing (1/8" OD x 1/16" ID PTFE tubing) are part of <strong>the</strong> accessory kit of <strong>the</strong><br />

instrument. Please add a suitable connec<strong>to</strong>r <strong>to</strong> <strong>the</strong> external pressure supply.<br />

The inlet for <strong>the</strong> external high pressure gas is situated at <strong>the</strong> back of <strong>the</strong><br />

instrument:<br />

136 <strong>Agilent</strong> <strong>7100</strong> <strong>Capillary</strong> <strong>Electrophoresis</strong> <strong>System</strong> User Manual

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