Introduction to the Agilent 7100 Capillary Electrophoresis System

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5 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Instrument Curves Using a further tab in the CE Method Setup dialog box you can choose to monitor and store the following CE-specific raw data. If selected, they are stored in addition to the detector signals defined: For the CE: • Leak current • Internal pressure • Replenishment level pressure • Vacuum • Injection pressure • External pressure • Tray temperature • Cassette temperature For the DAD: • Board temperature • Optical unit temperature • UV lamp anode voltage 106 Agilent 7100 Capillary Electrophoresis System User Manual
Using the Agilent 7100 Capillary Electrophoresis System 5 Capillary Electrophoresis, CE Detector Parameters The parameters for the detector are set in the DAD tab of the Method Setup dialog used in the Agilent ChemStation (see Figure 20 on page 93). On the left hand-side you can define the following settings: • Used signals, detection and reference wavelengths, bandwidths • Peak width or detector response time • Detector stoptime and posttime Signals, Wavelengths and Bandwidths Detector parameters allow you to define the detector signals you want to acquire during the analysis and store in the data file. For each signal the wavelengths and bandwidths of sample and reference wavelengths are defined. It is recommended to do runs without using reference wavelengths to minimize baseline noise. Reference wavelengths can help to compensate for signal drifts. As reference wavelengths are usually measured at higher wavelengths, the baseline noise is increased when using reference wavelengths. Therefore, it might be appropriate to record the two signals at the same wavelength; one using the reference wavelength and one without. The signal you want to store in the data file is displayed below the lamp icon of the DAD tab of the instrument diagram. Peak Width The peak width sets the optimum response time of your detector. If you have similar peak widths in your electropherogram, set it to the width of one of the peaks in your electropherogram. If the peak widths are very different you can use the detector timetable to adjust the peak width setting during the analysis. If your peak width setting is too narrow, peaks might be not detected automatically. This will lead to no spectra for the wider peaks being acquired and in case of peak dependent detection. Agilent 7100 Capillary Electrophoresis System User Manual 107
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Agilent 7100 Capillary Electrophore
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In This Guide… This guide contain
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Contents 1 Introduction 13 Introduc
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Contents Delivery Checklist 55 Inst
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Contents Running the CEC Analysis 1
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Contents 9 Parts and Materials for
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Introduction 1 Where to Find Inform
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Site Requirements and Specification
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Troubleshooting and Diagnostics 6 C
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Troubleshooting and Diagnostics 6 A
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Hardware Information 7 7100 Capilla
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Maintenance 8 Overview of Maintenan
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Maintenance 8 Cleaning the Electrod
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Maintenance 8 Maintenance of the Re
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Maintenance 8 Changing the Air Inle
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Appendix 10 General Safety Informat
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Appendix 10 Allgemeine Sicherheitsi
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Appendix 10 Allgemeine Sicherheitsi
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Appendix 10 Informations générale
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Appendix 10 Informazioni generali s
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Appendix 10 Información de segurid
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Appendix 10 安全に関する
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Appendix 10 安全に関する
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Appendix 10 一般安全信息
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Appendix 10 Setting Up a Test Sampl
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Appendix 10 The Waste Electrical an
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Appendix 10 Agilent Technologies on
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Index A accessing 199 accessories 5
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