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Introduction to the Agilent 7100 Capillary Electrophoresis System

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Using <strong>the</strong> <strong>Agilent</strong> <strong>7100</strong> <strong>Capillary</strong> <strong>Electrophoresis</strong> <strong>System</strong> 5<br />

How <strong>to</strong> Prepare your <strong>Agilent</strong> CE Instrument for Analysis<br />

How <strong>to</strong> Prepare your <strong>Agilent</strong> CE Instrument for Analysis<br />

To prepare <strong>the</strong> <strong>Agilent</strong> CE instrument for <strong>the</strong> analysis you need <strong>to</strong> do <strong>the</strong><br />

following tasks:<br />

• Start <strong>the</strong> <strong>Agilent</strong> CE instrument.<br />

• Start <strong>the</strong> computer.<br />

• Start <strong>the</strong> <strong>Agilent</strong> ChemStation software/CE user interface.<br />

• Allow <strong>the</strong> lamp <strong>to</strong> warm up (approximately 1 hour).<br />

• Prepare vials for sample and buffer.<br />

• Insert a capillary in<strong>to</strong> <strong>the</strong> alignment interface.<br />

• Insert <strong>the</strong> capillary in<strong>to</strong> <strong>the</strong> capillary cassette.<br />

• Insert <strong>the</strong> capillary cassette in<strong>to</strong> <strong>the</strong> system.<br />

• Prepare <strong>the</strong> replenishment system (optional).<br />

After you have completed all <strong>the</strong>se tasks, you can start an <strong>Agilent</strong> CE method.<br />

A CE method can be created in an online (CE instrument is on and connected)<br />

or offline installation of <strong>the</strong> <strong>Agilent</strong> ChemStation.<br />

The detec<strong>to</strong>r lamp can be switched on au<strong>to</strong>matically and unattended by<br />

scheduling this from <strong>the</strong> detec<strong>to</strong>r control menu. Please see <strong>the</strong> options for <strong>the</strong><br />

detec<strong>to</strong>r under “Diode Array Detec<strong>to</strong>r” on page 179.<br />

The detec<strong>to</strong>r lamp can be switched on au<strong>to</strong>matically with each instrument<br />

start. Select <strong>the</strong> respective setting from <strong>the</strong> detec<strong>to</strong>r control menu (see “Diode<br />

Array Detec<strong>to</strong>r” on page 179).<br />

<strong>Agilent</strong> <strong>7100</strong> <strong>Capillary</strong> <strong>Electrophoresis</strong> <strong>System</strong> User Manual 91

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