Introduction to the Agilent 7100 Capillary Electrophoresis System

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6 Troubleshooting and Diagnostics CE Problem Solving If the leak sensor is activated or you think there is a leak in the instrument: 1 Immediately switch off the Agilent CE instrument (and the external water bath, if connected). 2 Disconnect the line power cord. 3 Open the lid. 4 Remove the front cover as described to diagnose the cause of leak. 5 Dry the leak sensor using a tissue. NOTE The error will stay active for the ChemStation or Lab Advisor even after corrective action and drying. Please acknowledge the error by clicking "Clear error" in the Lab Advisor or switching the power knob in the top right corner of the CE diagram of the ChemStation. Possible Reasons for Leaks and Appropriate Action for Spilled Liquid from Other Sources • If you have spilled some liquid into the Agilent CE instrument, it is sufficient to thoroughly clean and dry the Agilent CE instrument and the leak sensor. Leaking Water Bath Tubing Inside the Agilent CE Instrument • Call Agilent Technologies. • Do not use the Agilent CE instrument until Agilent Technologies has repaired it. Leaks in Replenishment Tubing or Valves • Call Agilent Technologies. Reason Unknown • If you can not trace the reason for the leak, call Agilent Technologies. There may be an instrument problem. 164 Agilent 7100 Capillary Electrophoresis System User Manual
Troubleshooting and Diagnostics 6 CE Problem Solving Blocked Replenishment Needle If you are using the replenishment function of the Agilent CE instrument, buffer may precipitate inside the replenishment needle. To avoid this, put a vial filled with water or buffer (to a height of 1.8 cm) in vial position 49. Vial position 49 is the home position of the replenishment lift. The vial will be picked up by the lift and brought to the replenishment needle. The needle will dip into the water, thus preventing the buffer from precipitating. (see “Preparing Vials for Buffer and Sample” on page 115, Vial position 49) Leakage Current Leakage current is indicated by a yellow bar in the current part of the energy status display in the CE diagram. Only high leakage current will lead to this. An instrument curve allows you to monitor current leakage even at low values from the ChemStation. Leakage current means that the current at the inlet electrode is different from the one at the outlet electrode and thus a current flows outside the capillary. A certain leakage current must be regarded as acceptable, since it is not avoidable due to technical reasons. It can be caused by: • A broken capillary • An empty inject vial • An air bubble in injection vial/or run buffer vials • An air bubble in capillary • A contaminated insulation plate • A high humidity level • A conductive capillary surface If you find a broken capillary in the alignment interface, use an ultrasonic bath to clean the interface and remove quartz residues. Agilent 7100 Capillary Electrophoresis System User Manual 165
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Agilent 7100 Capillary Electrophore
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In This Guide… This guide contain
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Contents 1 Introduction 13 Introduc
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Contents Delivery Checklist 55 Inst
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Contents Running the CEC Analysis 1
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Contents 9 Parts and Materials for
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Introduction 1 Where to Find Inform
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Site Requirements and Specification
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Installing the Instrument 3 Unpacki
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Installing the Instrument 3 Install
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Installing the Instrument 3 Install
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LAN Configuration 4 TCP/IP Paramete
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LAN Configuration 4 Initialization
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LAN Configuration 4 Initialization
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LAN Configuration 4 Link Configurat
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LAN Configuration 4 Manual Configur
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LAN Configuration 4 Agilent Bootp S
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Controlling the Agilent CE Instrume
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Using the Agilent 7100 Capillary El
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Maintenance 8 Cleaning the Electrod
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Maintenance 8 Changing the Air Inle
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Appendix 10 General Safety Informat
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Appendix 10 Allgemeine Sicherheitsi
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Appendix 10 Allgemeine Sicherheitsi
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Appendix 10 Informations générale
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Appendix 10 Informazioni generali s
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Appendix 10 Información de segurid
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Appendix 10 安全に関する
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Appendix 10 安全に関する
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Appendix 10 一般安全信息
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Appendix 10 Setting Up a Test Sampl
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Appendix 10 Setting Up a Test Sampl
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Appendix 10 The Waste Electrical an
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Appendix 10 Sound Emission Sound Em
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Appendix 10 Agilent Technologies on
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Index A accessing 199 accessories 5
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www.agilent.com In This Book This m
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