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Introduction to the Agilent 7100 Capillary Electrophoresis System

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Controlling <strong>the</strong> <strong>Agilent</strong> CE Instrument<br />

Using <strong>the</strong> <strong>Agilent</strong> <strong>7100</strong> <strong>Capillary</strong> <strong>Electrophoresis</strong> <strong>System</strong> 5<br />

Controlling <strong>the</strong> <strong>Agilent</strong> CE Instrument<br />

Before You Start <strong>the</strong> <strong>Agilent</strong> CE Instrument<br />

Use <strong>the</strong> following as a checklist before you start <strong>the</strong> <strong>Agilent</strong> CE instrument.<br />

Make sure that:<br />

• <strong>the</strong> replenishment bottles are installed and properly tightened,<br />

• <strong>the</strong> cassette (with a capillary installed) is inserted in <strong>the</strong> system,<br />

• <strong>the</strong> cassette door and <strong>the</strong> tray door are closed, and<br />

• you do not need <strong>to</strong> put <strong>the</strong> vials in<strong>to</strong> <strong>the</strong> tray before you start <strong>the</strong><br />

<strong>Agilent</strong> CE instrument<br />

Starting Up <strong>the</strong> <strong>Agilent</strong> CE Instrument<br />

The power switch of <strong>the</strong> instrument is located on <strong>the</strong> left front corner of <strong>the</strong><br />

instrument.<br />

Press this switch <strong>to</strong> turn on <strong>the</strong> line power.<br />

After <strong>the</strong> instrument is started, <strong>the</strong> fans and <strong>the</strong> pump start working. The<br />

instrument starts <strong>to</strong> build-up internal pressure (approximately 950 mbar)<br />

first, followed by a vacuum. The pump becomes idle and switches on only<br />

occasionally once <strong>the</strong> pressure and vacuum setpoints are reached.<br />

During initialization <strong>the</strong> instrument tests different functions and adjustments.<br />

The tray as well as all three lift stations are moved.<br />

The UV lamp will switch on during instrument startup based on parameters<br />

that can be defined in <strong>the</strong> DAD control section, see “Diode Array Detec<strong>to</strong>r” on<br />

page 179. O<strong>the</strong>rwise, <strong>the</strong> lamp can be switched on from <strong>the</strong> DAD diagram of<br />

<strong>the</strong> ChemStation.<br />

The meaning of <strong>the</strong> LED colors on <strong>the</strong> front cover are explained in <strong>the</strong><br />

following table.<br />

<strong>Agilent</strong> <strong>7100</strong> <strong>Capillary</strong> <strong>Electrophoresis</strong> <strong>System</strong> User Manual 79

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