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Introduction to the Agilent 7100 Capillary Electrophoresis System

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8 Maintenance<br />

Overview of Maintenance<br />

Table 25<br />

Overview of maintenance<br />

Procedure Typical frequency Notes<br />

Repair of replenishment<br />

needle<br />

• Use <strong>the</strong> Clean Level Sensor task <strong>to</strong> remove salt<br />

deposits inside <strong>the</strong> tubing system.<br />

• Keep a water-filled parking vial in carousel<br />

position 49.<br />

• Occasionally replace <strong>the</strong> parking vial in position<br />

49 with water or buffer.<br />

• Inspect for bending if clogged.<br />

• Remove persistent clogging by a syringe flush<br />

with water/isopropanol or use ultrasonic<br />

device.<br />

Buffer reservoir • Inspect O-ring in cap for damage, remove salt<br />

deposits.<br />

• Do not use buffer reservoir for long-term<br />

s<strong>to</strong>rage (prevents oxygen saturation and<br />

bacteria from growing).<br />

• Remove liquid from waste bottle.<br />

Inlet frit • Inspect and clean (especially if absorbing<br />

buffers have been used).<br />

• Replace when dirty.<br />

Vial caps • Do not overuse vial caps (especially <strong>the</strong> PEO<br />

caps).<br />

• Inspect for damage.<br />

* The chosen frequency may be time-based, for example weekly or injection-based, for example 100 injections. It depends on<br />

your individual application and needs. The time and injection-based EMF counter can be cus<strong>to</strong>mized.<br />

NOTE<br />

Do not open <strong>the</strong> <strong>to</strong>p cover or remove <strong>the</strong> cartridge without lowering <strong>the</strong> lifts. Use <strong>the</strong><br />

function maintenance position from <strong>the</strong> <strong>to</strong>ols section of <strong>the</strong> Lab Advisor. Careless opening<br />

may lead <strong>to</strong> floating of electrodes with buffer or sample which leads <strong>to</strong> contamination or<br />

carry-over.<br />

192 <strong>Agilent</strong> <strong>7100</strong> <strong>Capillary</strong> <strong>Electrophoresis</strong> <strong>System</strong> User Manual

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