Introduction to the Agilent 7100 Capillary Electrophoresis System

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10 Appendix Setting Up a Test Sample Run Setting Up a Test Sample Run This instrument check demonstrates that the instrument is correctly installed and connected. Please use a fused silica capillary with 40 cm effective length and 50 µm inner diameter from the HPCE start-up capillary kit (part number G1600-68700) with the green alignment interface (part number G7100-60210). The instrument should be in CE mode. Load chemicals from the Installation Qualification Chemical kit (part number 5063-6514) according to the table below. The Installation Qualification Chemical kit contains: • Buffer (20 mM borate, pH 9.3, 100 mL) • Test sample (4-hydroxyacetophenone 1 mM, 3 mL) • Conditioning solution (1.0 N sodium hydroxide, 100 mL) Table 34 Solution for Checkout Run Location Solution Volume Vial 1 1.0 N sodium hydroxide 500 µL Vial 2 Water 200 µL Vial 4 Buffer 500 µL Vial 5 Buffer 500 µL Vial 6 Buffer 500 µL Vial 7 Test sample 500 µL Vial 8 Water/waste 200 µL Choose the menu item Method > New method to prepare a method with the parameters given below. Save the edited method under a new name. Choose the menu item RunControl > Sample Info… and enter the operator name, directory and file name. Enter Vial location 7, enter sample name “1 mM pHAP” and click Run method to start the analysis. A typical electropherogram will show a significant peak at a migration time of around 3 minutes. 252 Agilent 7100 Capillary Electrophoresis System User Manual
Appendix 10 Setting Up a Test Sample Run Method Parameter CE (Default Settings) Vials • Inlet home vial 4 • Outlet home vial 5 Cassette Temperature • 20.0°C High Voltage System • Enable high voltage • Voltage 0.0 V (will ramp up in accordance with timetable) • Current 300 µA • Power 6.0 W • Low current limit OFF Stoptime • 10.0 minutes Posttime • OFF Replenishment • Replenishment and preconditioning parallel processing • No replenishment used Agilent 7100 Capillary Electrophoresis System User Manual 253
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Agilent 7100 Capillary Electrophore
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In This Guide… This guide contain
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Contents 1 Introduction 13 Introduc
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Contents Delivery Checklist 55 Inst
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Contents Running the CEC Analysis 1
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Contents 9 Parts and Materials for
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Introduction 1 Where to Find Inform
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Site Requirements and Specification
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LAN Configuration 4 Initialization
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LAN Configuration 4 Agilent Bootp S
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Controlling the Agilent CE Instrume
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Using the Agilent 7100 Capillary El
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Troubleshooting and Diagnostics 6 A
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Hardware Information 7 7100 Capilla
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Maintenance 8 Overview of Maintenan
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Maintenance 8 Early Maintenance Fee
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Maintenance 8 Cleaning the Electrod
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Maintenance 8 Cleaning the Electrod
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Introduction to the Agilent 7100 Capillary Electrophoresis System

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