Introduction to the Agilent 7100 Capillary Electrophoresis System

5 Using the Agilent 7100 Capillary Electrophoresis System
Capillary Electrophoresis, CE
Spectra
On the right-hand side of the DAD Method Setup dialog an expandable page for
advanced settings is visible.
Under Spectrum you can define at which points on a signal, spectra will be
taken and saved. You can choose to acquire them:
• At the upslope, apex and downslope of the peak
• All in a peak
• All spectra during analysis
• Every 2nd spectrum
or
• None
You can combine this setting with a wavelength range. By restricting the
wavelength range to the range of your interest you can save some disk space;
the size of your data files will be smaller because the full spectrum is not
stored.
A peak height threshold avoids spectra that are taken from small peaks.
If spectra are recorded, a spectra icon is visible in the detector diagram of the
graphical user interface.
Detector Stoptime and Posttime
You can define a special stoptime and posttime for your detector. If you want
to use the times set for the Agilent CE instrument, set them to as CE.
Analog Output
The Analog Output section enables you to set up the zero offset and attenuation
of the analog output signal.
Indirect UV
The section Indirect UV enables you to set a margin for peaks with negative
absorbance. Set it as close to the baseline as possible but allowing enough
range for the negative peaks you expect to get. The higher the value, the lower
the resolution of the detector, and the greater the baseline noise. Set this value
only if you expect negative absorbances greater than -100 mAU.
108 Agilent 7100 Capillary Electrophoresis System User Manual