Introduction to the Agilent 7100 Capillary Electrophoresis System

More documents

Recommendations

Info

5 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrochromatography, CEC CEC Control Using the CE Diagram High pressure may be applied independently of the method for capillary conditioning or cleaning by clicking the external pressure icon in the CE diagram (see Figure 46 on page 152). This will cause a parameter setting field to appear (see Figure 47 on page 154). From this field you may chose to apply pressure to the inlet, outlet or both vials, and also whether to simultaneously apply a voltage of -30 to 30 kV for a given time. 158 Agilent 7100 Capillary Electrophoresis System User Manual
Using the Agilent 7100 Capillary Electrophoresis System 5 Capillary Electrochromatography, CEC Running the CEC Analysis After setting up the operational parameters, you can initiate the run from the Method & Run Control menu by clicking Run Method or by clicking Start in the CE diagram. Some other operational aspects should be noted when running CEC separations. Capillaries Buffers Injection Packed capillaries are very fragile and must be handled with great care. Offline purging of the capillary using an LC pump may be necessary if air bubbles form within the capillary. CEC buffers generally have lower concentrations (4–20 mM) than conventional CE buffers. These may also contain a larger proportion of organic modifier than is found in CZE or MECC buffers. The currents generated are generally on the order of 2–10 µA for an applied field of approximately 600 V/cm. Because of the resistance of the packed bed, the usual applied pressure of up to 50 mbar is insufficient to introduce sample onto the capillary. In this case, high pressure and/or electrokinetic loading may be used, although the limitations of this technique should be noted. Problem Solving Offline purging NOTE If air bubbles occur during an analysis, the current will become instable or very low (< 0.3 µA). If this occurs, remove the packed capillary and connect it to an LC pump capable of delivering a flow rate of 40 µL/min to the capillary. Great care should be taken when removing and handling the capillary and when connecting and disconnecting the capillary to or from the pump. Fused silica packed capillaries are extremely fragile. Instrument purging If the capillary is filled with solvent, then this may be replaced by inserting the capillary into the instrument and flushing with external pressure using the external gas supply icon on the CE diagram. Generally, 10–60 minutes are sufficient to replace the storage solvent with the operating mobile phase, depending on the velocity of EOF. Agilent 7100 Capillary Electrophoresis System User Manual 159
Page 1 and 2:
Agilent 7100 Capillary Electrophore
Page 3 and 4:
In This Guide… This guide contain
Page 5 and 6:
Contents 1 Introduction 13 Introduc
Page 7 and 8:
Contents Delivery Checklist 55 Inst
Page 9 and 10:
Contents Running the CEC Analysis 1
Page 11 and 12:
Contents 9 Parts and Materials for
Page 13 and 14:
Page 15 and 16:
Introduction 1 Where to Find Inform
Page 17 and 18:
Page 19 and 20:
Site Requirements and Specification
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Page 37 and 38:
Page 39 and 40:
Page 41 and 42:
Page 43 and 44:
Page 45 and 46:
Page 47 and 48:
Page 49 and 50:
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Installing the Instrument 3 Unpacki
Page 57 and 58:
Installing the Instrument 3 Install
Page 59 and 60:
Installing the Instrument 3 Install
Page 61 and 62:
Page 63 and 64:
LAN Configuration 4 TCP/IP Paramete
Page 65 and 66:
LAN Configuration 4 Initialization
Page 67 and 68:
LAN Configuration 4 Initialization
Page 69 and 70:
LAN Configuration 4 Link Configurat
Page 71 and 72:
LAN Configuration 4 Manual Configur
Page 73 and 74:
LAN Configuration 4 Manual Configur
Page 75 and 76:
LAN Configuration 4 Agilent Bootp S
Page 77 and 78:
Page 79 and 80:
Controlling the Agilent CE Instrume
Page 81 and 82:
Using the Agilent 7100 Capillary El
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92:
Page 93 and 94:
Page 95 and 96:
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108: Using the Agilent 7100 Capillary El
Page 157: Using the Agilent 7100 Capillary El
Page 161 and 162: Agilent 7100 Capillary Electrophore
Page 163 and 164: Troubleshooting and Diagnostics 6 C
Page 169 and 170: Troubleshooting and Diagnostics 6 A
Page 173 and 174: Hardware Information 7 7100 Capilla
Page 175 and 176: Hardware Information 7 7100 Capilla
Page 177 and 178: Hardware Information 7 Firmware Fir
Page 179 and 180: Hardware Information 7 Diode Array
Page 181 and 182: Hardware Information 7 Diode Array
Page 183 and 184: Hardware Information 7 Installing D
Page 185 and 186: Hardware Information 7 External Wat
Page 187 and 188: Hardware Information 7 Analog to Di
Page 191 and 192: Maintenance 8 Overview of Maintenan
Page 193 and 194: Maintenance 8 Overview of Maintenan
Page 195 and 196: Maintenance 8 Early Maintenance Fee
Page 197 and 198: Maintenance 8 Cleaning the Electrod
Page 209 and 210:
Maintenance 8 Cleaning the Electrod
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 217 and 218:
Maintenance 8 Maintenance of the Re
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Maintenance 8 Changing the Air Inle
Page 225 and 226:
Maintenance 8 Changing the Lamp Rem
Page 227 and 228:
Maintenance 8 Changing the Lamp Ins
Page 229 and 230:
Page 231 and 232:
Page 233 and 234:
Appendix 10 General Safety Informat
Page 235 and 236:
Appendix 10 Allgemeine Sicherheitsi
Page 237 and 238:
Appendix 10 Allgemeine Sicherheitsi
Page 239 and 240:
Appendix 10 Informations générale
Page 241 and 242:
Appendix 10 Informazioni generali s
Page 243 and 244:
Appendix 10 Informazioni generali s
Page 245 and 246:
Appendix 10 Información de segurid
Page 247 and 248:
Appendix 10 安全に関する
Page 249 and 250:
Appendix 10 安全に関する
Page 251 and 252:
Appendix 10 一般安全信息
Page 253 and 254:
Appendix 10 Setting Up a Test Sampl
Page 255 and 256:
Appendix 10 Setting Up a Test Sampl
Page 257 and 258:
Appendix 10 The Waste Electrical an
Page 259 and 260:
Appendix 10 Sound Emission Sound Em
Page 261 and 262:
Appendix 10 Agilent Technologies on
Page 263 and 264:
Index A accessing 199 accessories 5
Page 266:
www.agilent.com In This Book This m
show all

Introduction to the Agilent 7100 Capillary Electrophoresis System

Create successful ePaper yourself

Delete template?

Save as template?