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Introduction to the Agilent 7100 Capillary Electrophoresis System

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5 Using <strong>the</strong> <strong>Agilent</strong> <strong>7100</strong> <strong>Capillary</strong> <strong>Electrophoresis</strong> <strong>System</strong><br />

<strong>Capillary</strong> Electrochroma<strong>to</strong>graphy, CEC<br />

O<strong>the</strong>r parameters which can be set in <strong>the</strong> High pressure flush command menu<br />

include:<br />

• Applied voltage (kV), current (uA) or power (W)<br />

• Duration (s)<br />

• Inlet vial and/or outlet vial<br />

NOTE<br />

When applying voltage simultaneously, <strong>the</strong> inlet and oulet vials must contain electrolyte in<br />

order <strong>to</strong> maintain electrical contact.<br />

Injection Options with CEC<br />

The installed high pressure supply may also be used for injection purposes<br />

using <strong>the</strong> Timetable option in <strong>the</strong> Injection field of a method setup.<br />

From this menu you may select <strong>the</strong> High pressure flush command during<br />

injection with <strong>the</strong> same parameter choices as those available during<br />

preconditioning.<br />

This may be used <strong>to</strong> apply a pressure injection while using a replaceable gelfilled<br />

capillary.<br />

NOTE<br />

Please note that <strong>the</strong> external pressure is not as precise as <strong>the</strong> 50 mbar injection pressure!<br />

156 <strong>Agilent</strong> <strong>7100</strong> <strong>Capillary</strong> <strong>Electrophoresis</strong> <strong>System</strong> User Manual

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