Introduction to the Agilent 7100 Capillary Electrophoresis System

Maintenance 8
Maintenance of the Replenishment System
Changing the Buffer Composition in the Replenishment System
Please follow these steps.
1 Open the Lab Advisor or Instrument Utilities software and start monitoring
the respective instrument.
2 In the Tools section, select the CE Control screen and click on Change Bottles to
release the pressure before you open the bottles.
3 Take out the electrolyte bottle and unscrew the cap. The electrolyte bottle
has a frit and tubing inside. The frit must not get dirty.
4 Fill a minimum 300 mL of buffer into the electrolyte bottle. Make sure the
buffer is filtered by using a 0.25 µm or 0.45 µm filter depending on its
viscosity.
5 Make sure the waste bottle is empty.
6 Cap the bottles tightly.
7 Choose Done in the Change Bottles dialog box. Wait until the pressure has
built up again.
8 To flush the internal tubing system from the electrolyte bottle to the waste
bottle including all valves on this pathway, select Flush Tubes. During this
step the replenishment needle itself is not flushed. Set a flushing time of
0.3 minutes.
9 To clean the internal tubing system, including the replenishment, select
Clean Tubes. During this step the replenishment is used to fill and empty a
vial several times. Set the number of repetitions to 5.
10 When requested to do so, place a capped and empty vial into a tray position.
The involved volume of the replenishment system is about 5 mL. A 10-time
flush is sufficient. The flush time depends primarily on the viscosity of your
buffer. Therefore, choose a short time and repeat flush tubes if necessary,
otherwise it is possible that the buffer is flushed completely into the waste
bottle. This protocol suggests a combination of flush tubes first and a
subsequent clean tubes step, with comparably low volume consumption, for a
high exchange efficiency and low buffer consumption.
Agilent 7100 Capillary Electrophoresis System User Manual 217