Introduction to the Agilent 7100 Capillary Electrophoresis System

Using the Agilent 7100 Capillary Electrophoresis System 5
Capillary Electrophoresis, Plus High Pressure CE+p
Timetable controlled changes in the pressure settings are:
• OFF: Pressure usage is switched off.
• Internal, pressure (-100 mbar to +100 mbar): Can be applied to the inlet
vial.
• Flush: Standard flush procedure is applied with an internally generated
pressure of approximately 950 mbar.
• External: Allows pressurizing the inlet vial and/or the outlet vial. External
pressure is reduced to the set value between 2 and 12 bar and applied in a
controlled manner.
• Raw External Pressure: Allows pressurizing the inlet vial, and/or the outlet
vial. The external pressure up to 15 bar can be applied directly without
reduction.
CE+p Control Using the CE Diagram
High pressure may be applied independently of the method for capillary
conditioning or cleaning by clicking the external pressure icon in the CE
diagram (see Figure 39 on page 142). This will cause a parameter setting field
to appear (see Figure 41 on page 145). From this field you may chose to apply
pressure to the inlet, outlet or both vials, and also whether to simultaneously
apply a voltage of -30 to 30 kV for a given time.
Agilent 7100 Capillary Electrophoresis System User Manual 147