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Introduction to the Agilent 7100 Capillary Electrophoresis System

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5 Using <strong>the</strong> <strong>Agilent</strong> <strong>7100</strong> <strong>Capillary</strong> <strong>Electrophoresis</strong> <strong>System</strong><br />

<strong>Capillary</strong> <strong>Electrophoresis</strong>, CE<br />

Installing a <strong>Capillary</strong> in <strong>the</strong> Alignment Interface<br />

The following procedure describes how <strong>to</strong> install a capillary in<strong>to</strong> an alignment<br />

interface. A capillary must have an alignment interface installed before it can<br />

be installed in<strong>to</strong> <strong>the</strong> capillary cassette in order <strong>to</strong> align <strong>the</strong> inner capillary<br />

with <strong>the</strong> light path of <strong>the</strong> diode array detec<strong>to</strong>r<br />

Material Needed <strong>to</strong> Install a <strong>Capillary</strong> in <strong>the</strong> Alignment Interface<br />

• <strong>Capillary</strong><br />

• Corresponding alignment interface<br />

• <strong>Capillary</strong> insertion <strong>to</strong>ol (separate item) or part incorporated in cassette<br />

• Safety glasses<br />

WARNING<br />

Risk of injury of sharp capillary ends.<br />

Sharp capillary ends can injure your eyes or skin.<br />

• Wear safety glasses when opening <strong>the</strong> cassette and handling <strong>the</strong> capillary <strong>to</strong><br />

protect your eyes and body.<br />

NOTE<br />

Alignment interfaces for <strong>the</strong> <strong>7100</strong> CE instrument differ from alignment interfaces for earlier<br />

instruments. Interfaces from earlier instruments do not fit in<strong>to</strong> <strong>the</strong> diode array detec<strong>to</strong>r<br />

block.<br />

Color Coding of Capillaries and Alignment Interfaces<br />

The alignment interface must correspond <strong>to</strong> <strong>the</strong> type of capillary used. There<br />

are special alignment interfaces for straight capillaries and for extended light<br />

path capillaries. The alignment interface must also correspond <strong>to</strong> <strong>the</strong> inner<br />

diameter of <strong>the</strong> capillary. To make it easier <strong>to</strong> match <strong>the</strong> capillary and<br />

corresponding alignment interface, we use <strong>the</strong> following color coding on both<br />

<strong>the</strong> sleeve of <strong>the</strong> alignment interface and <strong>the</strong> plastic s<strong>to</strong>p on <strong>the</strong> capillary.<br />

124 <strong>Agilent</strong> <strong>7100</strong> <strong>Capillary</strong> <strong>Electrophoresis</strong> <strong>System</strong> User Manual

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