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P-25 Improving sustainable water use in grape production using a Drought Biomarker Detection (DBD) tool C.A. Vargas and S.T. Lund* Wine Research Centre Faculty of Land and Food Systems, University of British Columbia, Vancouver, Canada *Corresponding author: steven.lund@ubc.ca New technologies for improving water use efficiency are especially important for agricultural sectors. In the Okanagan Valley of British Columbia, agriculture accounts for 70% of total water use, with wine growing being the third major consumer. Other grape and wine growing regions such as California and Australia are facing similar water use issues. We are currently developing a device with the specific goal of helping to improve water use efficiency by the grape and wine industries. Although our primary focus is vineyards in the Okanagan region, the technology we are developing is anticipated to have applications to other regions and crops worldwide. Grape and wine growers rely largely on physiological markers such as visible wilting to diagnose water stress. Incorrect diagnoses can lead to over-watering and wastage of water resources, while delayed irrigation can result in temporary reductions in vine productivity or permanent vine damage. The DBD tool is envisioned to screen for biomarkers sensitive to changes in stem water potential but detectable before physiological symptoms are evident. Biomarkers are molecular factors (genes, proteins, or chemical compounds) that indicate physiological states at specific times under a defined set of conditions. The DBD is based on specific sandwich antibody detection adapted to a lateral immuno-chromatographic strip type of format, where a colored latex particle-antibody conjugate captures a drought specific biomarker, carries it along a cellulose membrane by capillary action until the free ends of the biomarker bind to the capture antibody and generates a colorful signal indicating a positive result, suggesting that the vine is healthy but irrigation is needed. If the test indicator does not reveal color, the diagnosis is that preventive irrigation is not required. The DBD will assist viticulturists to make better decisions concerning irrigation and is expected to be an improvement over current water stress diagnostic practices in its accuracy, cost, and ease of use. The DBD will contain as many reusable parts as possible to maximize sustainability and safe ease of use directly in the vineyard with minimal manipulation by the viticulturist. 101 
P-26 Identification of ABA inducible genes from cold-hardy Vitis amurensis Rupr. Cv. Zuoshan- 1 by suppression subtractive hybridization Y. Zhang* 1.2 , J. Wu 1 , H. Zhang 1 , J. Lu 1,2 1 College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China; 2 Center for Viticulture and Small Fruit Research, Florida A&M University, Tallahassee, Florida, USA *Corresponding author: olivia.yl.zhang@gmail.com Vitis amurensis Rupr., distributed in the Northeast of China, is the most cold hardy grape species. It can tolerate temperatures as low as -40-50 C when fully cold acclimated. Our study showed that as the temperature drops, the cold hardy“Zuoshan-1”grapevine accumulates ABA quicker and in higher concentration than less cold hardy Vitis species. To better understand the molecular mechanisms of ABA and its role in the grape cold hardiness, we constructed a suppression subtractive hybridization (SSH) cDNA library to isolate ABA inducible genes of “Zuoshan-1”. In a total of 214 randomly picked clones, 167 were successfully sequenced from the ABA-induced library, from which 31 contigs were assembled. Blasting analysis revealed that14 contigs were homologous to Vitis or plant species while the other 17 contigs showed high homology to Escherichia coli, which were apparently resulted from E.coli contamination. Functional analysis indicated that the putative functions of these contigs were associated with photosynthesis, cell defense or detoxification, and signal transduction. 102 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
Page 49 and 50: O-30 Annette Nassuth Function of Vi
Page 51 and 52: 10 to 25% of the chlorotic symptoms
Page 53 and 54: O-34 Cloning and characterization o
Page 55 and 56: O-36 New insight into the genetics
Page 57 and 58: O-38 Co-Localization of QTLs for Se
Page 59 and 60: O-40 Quantitative analysis of textu
Page 61 and 62: O-42 Transcriptional changes in res
Page 63 and 64: O-44 The Turkish grape (Vitis vinif
Page 65 and 66: Association genetics in relation wi
Page 67 and 68: O-47 Intravarietal variability of C
Page 69 and 70: O-49 Molecular survey of Georgian t
Page 71 and 72: O-51 Marker-assisted breeding: Iden
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77 and 78: P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99: P-24 The development of molecular m
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111 and 112: P-36 Looking for genetic polymorphi
Page 113 and 114: P-38 Characterization of a new horm
Page 115 and 116: P-40 Towards a characterization of
Page 117 and 118: the overall identified polymorphism
Page 119 and 120: P-43 Proteomic characterization of
Page 121 and 122: P-45 Preliminary observations on th
Page 123 and 124: grapevine which will eventually be
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
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P-71 Viticultural performance of so
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P-73    Molecular characterizat
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P-75 Variation of berry polyphenoli
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P-77 Morphological and molecular id
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P-79 Studies on an excellent, early
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P-81 Effectiveness of different cul
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P-83 Some early maturing table grap
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P-85 New triploid seedless table gr
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P-87 Grape breeding and viticulture
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P-89 The usefulness of allotetraplo
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P-91 Drying rate of fresh berries f
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P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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