conference schedule and program with abstracts - Horticulture ...

P-75
Variation of berry polyphenolic profiles of USDA Vitis vinifera germplasm
Z. Liang 1* , C. Owens 2 , G.-Y. Zhong 2 , L. Cheng 1
1 Department of Horticulture, Cornell University, Ithaca, New York, USA; 2 USDA-ARS Grape
Genetics Research Unit, Geneva, New York, USA
*Corresponding author: zl249@cornell.edu
Polyphenolics are an important class of secondary metabolites with significant benefits to human
nutrition and health. As a part of the effort to characterize USDA Vitis germplasm, we evaluated
the polyphenolic profiles in the berry samples of 347 Vitis vinifera cultivars, maintained at the
USDA Vitis Clonal Repository at Davis, CA, for two successive years. A total of 36 phenolic
compounds were identified via HPLC-MS and quantified by HPLC-DAD, including 16
anthocyanins, 2 hydroxybenzoic acids, 6 hydroxycinnamic acids, 6 flavonols and 6 flavanols.
The total anthocyanins ranged from 0 to 5.12 mg/g FW in 2008, and from 0 to 5.02 mg/g FW in
2009. The means of total anthocyanins in red cultivars were 1.47 and 1.49 mg/g FW,
respectively, in the two years. Malvidin 3-O-glucoside and malvidin 3-O-(6-O-coumaryl)-
glucoside were the most abundant anthocyanins, accounting for 60% of the total anthocyanins.
Anthocyanin concentrations were significantly higher in wine grapes than in table grapes in red
cultivars. Hydroxycinnamic acids ranged from 0.01 to 0.93, and from 0.01 to 0.87 mg/g FW in
the two years, with caftaric acid being the most abundant (accounting for 70% of the total). The
total hydroxycinnamic acids in wine grapes were significantly higher than in table grapes.
Flavanols ranged from 0.004 to 1.42 and 0.04 to 1.92 mg/g FW, respectively, in the two years,
with procyanidin B1 being the most abundant (accounting for 50% of the total). Flavonols
ranged from 0.01 to 0.2 in both years, with quercetin 3-O-glucuronide and quercetin 3-Oglucoside
being the most abundant. Hydroxybenzoic acids were the least (