conference schedule and program with abstracts - Horticulture ...
conference schedule and program with abstracts - Horticulture ...
conference schedule and program with abstracts - Horticulture ...
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
P-18<br />
Use of molecular markers for seedlessness in grapevine breeding<br />
M. Akkurt*, A. Cakir, M. Shidfar, A. Ergul, G. Söylemezoglu<br />
Ankara University, Faculty of Agriculture, Turkey<br />
*Corresponding author: akkurt@agri.ankara.edu.tr<br />
Molecular markers have been utilized for early selection of seedless grapevine cultivars in a<br />
grapevine breeding <strong>program</strong> of the Department of <strong>Horticulture</strong>, Faculty of Agriculture, Ankara<br />
University. A total of 415 F1 genotypes belonging to Alphonse Lavallee x Sultani hybrid<br />
combination were screened <strong>with</strong> SCC8 (Lahogue et al. 1998), SCF27 (Mejia <strong>and</strong> Hinrichsen<br />
2003) <strong>and</strong> VMC7F2 (Cabezas et al) markers. The entire 415 F1 genotypes revealed amplification<br />
products of expected sizes when amplified <strong>with</strong> SCC8 <strong>and</strong> genotypes showing SCC8+/SCC8+<br />
homozygote allele pattern from the products restricted <strong>with</strong> the Bgl II restriction enzyme were<br />
selected as c<strong>and</strong>idate genotypes for seedlessness. A total of 81 genotypes were selected using<br />
SCF27, possessing 2.0 kb b<strong>and</strong>s were also selected as c<strong>and</strong>idates. Finally, genotypes possessing<br />
198 bp allele when amplified <strong>with</strong> VMC7F2 were selected as c<strong>and</strong>idates. As a result of the<br />
marker assisted selection studies, putative genotypes were selected for use in breeding studies for<br />
seedlessness as parental lines in future.<br />
94