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O-35 QTL analysis of predatory mite abundance and leaf morphology traits in a hybrid grape population C.L. Owens 4 , B.I. Reisch 1 , H. Schwaninger 3 , M. Aradhya 5 , C. Simon 3 , S. Mahanil 4 , R. Loughner 2 , K. Wentworth 2 , G. Loeb 2 , J. Nyrop 2 1 Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, Geneva, New York, USA; 2 Department of Entomology, New York State Agricultural Experiment Station, Cornell University, Geneva, New York, USA; 3 USDA-ARS, Plant Genetic Resources Unit, Geneva, New York, USA; 4 USDA-ARS, Grape Genetics Research Unit, Geneva, New York, USA; 5 USDA-ARS, National Clonal Germplasm Repository, Davis, California, USA *Corresponding author: clo5@cornell.edu Non-glandular leaf trichomes and domatia in grapes have been shown to positively influence the abundance of several species of predatory phytoseiid mites that feed on spider mite pests. These leaf structures, therefore, indirectly contribute to plant defense. Predatory mite abundance and several leaf morphological traits were measured in a grapevine population segregating for these morphological traits and regions of the genome underlying these traits was determined through QTL analysis. A genetic linkage map was constructed from 171 progeny of the cross of two interspecific hybrids, Ill. 547-1 (V. rupestris x V. cinerea) and ‘Horizon’, using 1068 molecular markers. Phytoseiid mite abundance was determined for 4 years and leaf morphological traits were measured in three growing seasons. The morphological traits measured were: leaf width, leaf length, vein bristles, vein hairs, domatia size, blade bristles, and blade hairs. Domatia size is the diameter of tufts of trichomes in vein axils; bristle and hair measurements estimate the density of shorter (
O-36 New insight into the genetics of color in grape A. Fournier-Level* 1 , L. Le Cunff 2 , A. Doligez 1 , P. Hugueney 4 , J.M. Souquet 4 , V. Cheynier 4 , A. Ageorges 4 , P. This 1 1 UMR DIAPC INRA-Montpellier Supagro, Montpellier, France; 2 UMT GenoVigne, IFV- INRA-Montpellier Supagro, Montpellier, France; 3 UMR SVQV, INRA Colmar, Colmar, France; 4 UMR Sciences pour l’oenologie INRA-Montpellier Supagro, Montpellier, France *Corresponding author: Alexandre_Fournier-Level@brown.edu Performing combined QTL and association mapping studies using the combination of synthetic lines and natural diversity represents an opportunity to develop the full picture of the genetic variation for quantitative traits and ultimately to identify the causal mutations. This approach was performed in order to decipher the genetic architecture of anthocyanin composition in berry skin, a crucial trait for both wine quality and human nutrition. Grapes may either be white or colored, ranging from the lightest pink to the darkest purple tones depending on the amount of anthocyanins accumulated in the berry skin. Based on the study of total content of anthocyanins in the skin of mature berries of both a segregating population of 191 progenies and a core collection of natural resources (141 individuals), we identified 2 QTLs for the traits accounting for 62% and 7.1% of the total variation, respectively. With the positional information provided through this QTL mapping, we identified in both cases Myb candidate genes and identified SNPs markers in high linkage with total content of skin anthocyanins. Using a multivariate regression method, we demonstrated that six polymorphisms accounted for 86% of the observed variation. All these polymorphisms either led to structural changes in the MYB proteins or differences in the VvMybAs promoters. Colour tones of skin are not only due to the overall pigment concentration but also to the presence of different types of pigments and in particular the level of methylation. A similar analysis was performed for the level of anthocyanin methylation in skin anthocyanins. Two QTLs were identified accounting for 37% and 17% of the trait variation respectively. Association mapping, transcriptomic analysis and enzymatic tests enabled the identification of two mutations in a structural gene functionally linked to the difference in methylation of anthocyanins. The use of natural diversity thus helped to reduce QTL to a set of QTNs, gave a clearer picture of how isogenes combined their effects to shape the quantitative traits, and revealed the relative influence of different cis-and trans-regulatory elements on these quantitative traits. 56 
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Date Time Session Topic Sunday Augu
Page 3 and 4: Monday August 2, 2010 Opening Plena
Page 5 and 6: Topic: Adaptation to soils and clim
Page 7 and 8: Tuesday August 3, 2010 Oral Session
Page 9 and 10: Poster Session 2 1:30 PM-3:00 PM Po
Page 11 and 12: P-105 Pathogen responsiveness and v
Page 13 and 14: Table of Contents Keynote Presentat
Page 15 and 16: K-2 Progress in grapevine breeding
Page 17 and 18: K-4 Genetics and Genomics of Grapev
Page 19 and 20: O-2 Additional homologs of the Ren1
Page 21 and 22: O-4 Y. Xu and Y.Wang* Introduction
Page 23 and 24: O-5 Identification of an R2R3 MYB t
Page 25 and 26: O-7 Sequencing of the phylloxera re
Page 27 and 28: O-9 Dissection of defense pathways
Page 29 and 30: O-11 Recent trends and technologies
Page 31 and 32: O-13 Brazilian Grape Breeding Progr
Page 33 and 34: O-15 Development of rootstocks for
Page 35 and 36: O-17 L. Wang* 1 , S. Li 1, 2 , P. F
Page 37 and 38: O-19 Integrated analysis of transcr
Page 39 and 40: O-20 Deciphering the chromosome str
Page 41 and 42: O-22 Identification of table grapes
Page 43 and 44: O-24 Metagenomic sequencing as a to
Page 45 and 46: O-26 Regulation of bud fruitfulness
Page 47 and 48: O-28 Haplotype structure and cluste
Page 49 and 50: O-30 Annette Nassuth Function of Vi
Page 51 and 52: 10 to 25% of the chlorotic symptoms
Page 53: O-34 Cloning and characterization o
Page 57 and 58: O-38 Co-Localization of QTLs for Se
Page 59 and 60: O-40 Quantitative analysis of textu
Page 61 and 62: O-42 Transcriptional changes in res
Page 63 and 64: O-44 The Turkish grape (Vitis vinif
Page 65 and 66: Association genetics in relation wi
Page 67 and 68: O-47 Intravarietal variability of C
Page 69 and 70: O-49 Molecular survey of Georgian t
Page 71 and 72: O-51 Marker-assisted breeding: Iden
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77 and 78: P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
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P-30 In silico SNP detection for ge
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P-32 Vitis vinifera genome annotati
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P-34 Genome-wide identification of
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P-36 Looking for genetic polymorphi
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P-38 Characterization of a new horm
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P-40 Towards a characterization of
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the overall identified polymorphism
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P-43 Proteomic characterization of
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P-45 Preliminary observations on th
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grapevine which will eventually be
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P-48 ‘Cioutat’: a somatic varia
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P-50 Estimation of protein spot var
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glucosyltransferase, anthocyanidin
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P-53 Marker-free transgenic grapevi
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P-55 Agrobacterium rhizogenes-media
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P-57 Phenotypic evaluation of ‘Th
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P-59 Phenotypic divergence among gr
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P-61 Grepevine variety determinatio
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agricultural context. This network
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P-64 Genetic structure in a large r
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P-66 Analysis of grape rootstocks b
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P-68 Italian wild grapevine: a stat
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Rebula-Robola group were genotyped
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P-71 Viticultural performance of so
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P-73    Molecular characterizat
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P-75 Variation of berry polyphenoli
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P-77 Morphological and molecular id
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P-79 Studies on an excellent, early
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P-81 Effectiveness of different cul
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P-83 Some early maturing table grap
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P-85 New triploid seedless table gr
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P-87 Grape breeding and viticulture
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P-89 The usefulness of allotetraplo
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P-91 Drying rate of fresh berries f
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P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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