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P-52 Establishment of virus-resistance in grapevine rootstocks G. Buchholz, M. Krieger, T. Manthey, C. Dubois, M. Sinn, J. Kramm, G.M. Reustle* RLP AgroScience GmbH, AlPlanta, - Institute for Plant Research, Neustadt / W, Germany *Corresponding author: goetz.reustle@agroscience.rlp.de Grapevine is affected by a multitude of viruses, leading to a loss of yield and quality of the grapes. The most destructive virus diseases worldwide are grapevine fanleaf and grapevine leafroll. An effective and sustainable protection against harmful viruses is to breed resistant elite and rootstock varieties. However there is no natural source of resistance available to be introduced by classic crossbreeding programmes. For this reason, RLP AgroScience is applying transgenic strategies (induction of gene silencing, expression of scFv-antibodies) to establish virus resistance both in elite and in rootstock varieties. In a project funded by the EU (FP5) and in cooperation with the Federal Association of Vine Plant Producers and a grapevine nursery, various gene constructs were developed, expressing i) the core region of the movement protein, ii) inverted repeats and iii) scFv-antibodies, in order to establish virus resistance against nepoviruses (GFLV, ArMV, RpRSV). Almost 100 transgenic lines of the rootstocks (SO4, Binova, 125AA, Richter 110) containing the different gene constructs have since been developed. The complete integration of the constructs has been confirmed in most of the transgenic lines by molecular characterisation. Between one and five copies of the transgenes were detected. In Northern analysis, transcripts of the relevant sequences were found in some but not in all transgenic lines. Detection of siRNA resulting from RNA-silencing of the virus-derived sequences is in process. Virus resistance of the transgenic lines is evaluated by laboratory screening methods, developed at AgroScience (dual culture of nematodes and candidate lines, detection of silencing by the GFP-sensor). Furthermore, transgenic lines are cultivated in a Saran-house equipped with soil containers, which were inoculated with Grapevine Fanleaf Virus (GFLV)-infected nematodes Xiphinema index. An overview about molecular analysis and the evaluation experiments for virus resistance of the transgenic lines will be presented. 131 
P-53 Marker-free transgenic grapevines using the Cre/lox recombination system G. Buchholz*, M. Sinn, T. Manthey, C. Dubois, G.M. Reustle RLP AgroScience GmbH, AlPlanta, - Institute for Plant Research, Neustadt / W, Germany *Corresponding author: guenther.buchholz@agroscience.rip.de The production of transgenic plants usually requires the application of selectable marker genes, enabling the specific selection of genetically modified cells and their regeneration to intact plants. Antibiotic resistance marker genes (ARMG) generally used for selection are insignificant for the practical use of the plants. In the scope of a network project funded by the Federal Ministry of Education and Research (BMBF) on the subject “optimisation of biological safety of transgenic plants”, the Cre/lox recombination system of the bacteriophage P1 was tested by RLP AgroScience to eliminate the ARMG from grapevine. For this reason, a vector system was developed allowing the removal of the ARMG by induced activity of the Cre-recombinase after transformation and regeneration. The essential feature of the construct is the concerted removal of the co-localised ARMG and cre-Gene cassettes from the genome by a sequence-specific recombination event. For the easy detection of a successful recombination, the coding sequence of a grapevine myb-transcription factor was inserted as a "gene of interest". In the case of recombination, this myb-factor activates the biosynthesis of anthocyanin, resulting in red coloured cells. The proof-of-concept was verified both in the model plant tobacco and in embryogenic grapevine cell culture. 132 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
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O-40 Quantitative analysis of textu
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O-42 Transcriptional changes in res
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O-44 The Turkish grape (Vitis vinif
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Association genetics in relation wi
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O-47 Intravarietal variability of C
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O-49 Molecular survey of Georgian t
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O-51 Marker-assisted breeding: Iden
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O-53 An Integrated approach to stud
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O-55 Characteristics of promising m
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P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111 and 112: P-36 Looking for genetic polymorphi
Page 113 and 114: P-38 Characterization of a new horm
Page 115 and 116: P-40 Towards a characterization of
Page 117 and 118: the overall identified polymorphism
Page 119 and 120: P-43 Proteomic characterization of
Page 121 and 122: P-45 Preliminary observations on th
Page 123 and 124: grapevine which will eventually be
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129: glucosyltransferase, anthocyanidin
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
Page 159 and 160: P-79 Studies on an excellent, early
Page 161 and 162: P-81 Effectiveness of different cul
Page 163 and 164: P-83 Some early maturing table grap
Page 165 and 166: P-85 New triploid seedless table gr
Page 167 and 168: P-87 Grape breeding and viticulture
Page 169 and 170: P-89 The usefulness of allotetraplo
Page 171 and 172: P-91 Drying rate of fresh berries f
Page 173 and 174: P-93 Development of table and raisi
Page 175 and 176: P-95 Reaction of grape rootstocks t
Page 177 and 178: P-97 Genetic analysis of the resist
Page 179 and 180: P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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