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P-56 Multifactorial analysis of fungal tolerance in genetically modified grapevine plants: defining prototypes for commercial purposes J. Rubio 1 , E. Tapia 3 , E. Sánchez 3 , A. Castro 3 , C. Montes 3 , MªA. Miccono 3 , P. Barba 3* , C. Álvarez 3 , B. Olmedo 3 , M. Muñoz 3 , L. Dalla Costa 2 , L. Martinelli 2 , H. Prieto 3 1 Programa Magister en Ciencias Agropecuarias, Facultad de Ciencias Agronomicas, Universidad de Chile, Santiago, Chile; 2 Fondazione Edmund Mach-IASMA Research and Innovation Centre, Genomics and Crop Biology Area. San Michele all’Adige, Trento, Italy; 3 Laboratorio de Biotecnología, Instituto de Investigación Agropecuaria, CRI – La Platina, Santiago, Chile *Corresponding author: pbarba@gmail.com Botrytis cinerea is a necrotrophic fungus that infects a large variety of horticultural crops producing "grey mold" disease and significant economic damage. Chitinases are enzymes that catalyze the hydrolysis of sugars and in fungi have a role in the growth of hyphae and differentiation. In plants, which lack chitin, these enzymes form a defense system against fungal pathogens. A 42 kDa chitinase has been purified from Trichoderma atroviride (ex T. harzianum) and its gene has been widely used in genetic transformation of various plant species leading to the generation of genetically modified (GM) plants that show tolerance against Botrytis infection. We have previously developed several GM grapevine Thompson Seedless plants and released them into a biosafety field. After three seasons of evaluations (2006-2008), the statistical analysis of the results in leaf-challenges by Botrytis allowed the identification of a select group of plants with a resistant phenotype. Within these lines, those bearing fruits were further analyzed for their ability as a botry-static agent in inhibition-of-fungal-growth experiments on potato-dextrose-agar (PDA) dishes. Inhibition of the radial growth and changes in the hyphae morphogenesis were observed, showing that berry juices from GM plants have an important ability inhibiting the regular radial fungal growth when compared to patterns obtained in control PDA dishes, PDA+ autoclaved GM berry juices and PDA + non-GM control juices. Results indicate the presence of a thermo-labile component in GM berry juices that inhibits growth of Botrytis. 135 
P-57 Phenotypic evaluation of ‘Thompson Seedless’ grapes transformed with AtNHX1 growing in hydroponics and potted soils M. Venier 1* , A. Bermejillo 1 , M.F. Filippini 1 , S.F. Alonso 1 , C. Agüero* 1 , A. Dandekar 2 , E. Blumwald 2 1 Facultad de Ciencias Agrarias, Universidad Nac. de Cuyo, Luján de Cuyo, Mendoza, Argentina; 2 Department of Plant Sciences, University of California, Davis, One Shield Ave, Davis, California, USA *Corresponding author: cbaguero@ucdavis.edu Overexpression of vacuolar Na + /H + antiport AtNHX1 has been reported to improve plant salt tolerance. We produced transgenic plants of Vitis vinifera cv. ‘Thompson Seedless’ via Agrobacterium tumefaciens carrying the gene encoding for AtNHX1 under the control of the constitutive promoter CaMV35S. Independent transgenic lines with improved tolerance of salt stress were selected in first round evaluations. The objective of this study was to analyze the response of the selected lines to NaCl stress in hydroponics and potted soils. Untransformed and transgenic plants were acclimated to greenhouse conditions and grown hydroponically using Long Ashton nutrient solution. In the salinity treatments, NaCl concentrations were increased stepwise by 25 mM every 10 days up to 150 mM. In addition, untransformed and transgenic plants were grown in a mix of 7-3 grape pomace-sand soil and watered with Long Ashton nutrient solution with and without increasing concentrations of NaCl. Analysis of plant growth variables confirmed the improved tolerance of the transgenic lines, which showed higher shoot length, leaf number, leaf area, and fresh and dry weight of shoots and roots. However, their better performance was not correlated with higher Na accumulation in root and/or shoot tissue compared to the untransformed controls. Na and Cl tissue contents were higher in plants grown in hydroponics; and roots accumulated 1.5-2.5 times more NaCl than aerial parts. 136 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
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O-40 Quantitative analysis of textu
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O-42 Transcriptional changes in res
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O-44 The Turkish grape (Vitis vinif
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Association genetics in relation wi
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O-47 Intravarietal variability of C
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O-49 Molecular survey of Georgian t
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O-51 Marker-assisted breeding: Iden
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O-53 An Integrated approach to stud
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O-55 Characteristics of promising m
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P-2 PMEI and SPY: Two genes with po
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P-4 Gene-assisted selection for tab
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P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111 and 112: P-36 Looking for genetic polymorphi
Page 113 and 114: P-38 Characterization of a new horm
Page 115 and 116: P-40 Towards a characterization of
Page 117 and 118: the overall identified polymorphism
Page 119 and 120: P-43 Proteomic characterization of
Page 121 and 122: P-45 Preliminary observations on th
Page 123 and 124: grapevine which will eventually be
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133: P-55 Agrobacterium rhizogenes-media
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
Page 159 and 160: P-79 Studies on an excellent, early
Page 161 and 162: P-81 Effectiveness of different cul
Page 163 and 164: P-83 Some early maturing table grap
Page 165 and 166: P-85 New triploid seedless table gr
Page 167 and 168: P-87 Grape breeding and viticulture
Page 169 and 170: P-89 The usefulness of allotetraplo
Page 171 and 172: P-91 Drying rate of fresh berries f
Page 173 and 174: P-93 Development of table and raisi
Page 175 and 176: P-95 Reaction of grape rootstocks t
Page 177 and 178: P-97 Genetic analysis of the resist
Page 179 and 180: P-99 Berry cracking caused powdery
Page 181 and 182: P-101 Elicitation of grapevine defe
Page 183 and 184: P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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