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P-108 The genetic mapping of Xiphinema index resistance derived from Vitis arizonica by using SSR markers S. Van Zyl* 1,2 , M.A. Vivier 1 , S. Riaz 2 , M.A. Walker 2 1 Department of Viticulture and Oenology, Faculty of AgriSciences, Stellenbosch University, Matieland, South Africa; 2 Department of Viticulture and Enology, University of California, Davis, California, USA *Corresponding author: sonetvz@gmail.com A breeding program to develop rootstocks with resistance to Xiphinema index, the dagger nematode vector of grapevine fanleaf virus (GFLV), has been underway for many years at UC Davis. Strong resistance to X. index was discovered in a hybrid form of Vitis arizonica/girdiana (b42-26). Inheritance studies found its resistance to be controlled by a single dominant locus, which was later genetically mapped and named XiR1. In this study we are examining X. index resistance derived from a pure form of V. arizonica b40-14 that was collected from Chihuahua, Mexico. A mapping population (0705) was derived from the susceptible rootstock 161-49C crossed with 8916-22 (V. rupestris A. de Serres x b40-14), which is heterozygous for X. index resistance. Preliminary greenhouse screening results suggest a 1:1 resistant to susceptible segregation ratio for X. index in this population. To develop a genetic map, over 400 SSR markers were tested on a small set of genotypes that included the parents and a few progeny. A total of 190 markers were polymorphic for either or both parents, covering all 19 linkage groups. One hundred and forty of the polymorphic markers have been completed on the entire set of 187 progeny in the 0705 population. The addition of more SSR markers to saturate the linkage groups is underway. A moderately saturated genetic map will be used to identify the genomic regions and markers associated with X. index resistance that could be used for marker-assisted breeding programs and as a foundation for fine-scale mapping. 189 
P-109 Characterization of an ankyrin-like protein up-stream region from Vitis vinifera is highly induced by Botrytis cinerea infection M.A. Miccono 1 , M. Ortega 2 , C. Montes 1 , P. Barba* 1 , J. Rubio 1 , H. Peña-Cortés 3 , H. Prieto 1 1 Biotechnology Laboratory, INIA-La Platina Station, La Platina, Chile; 2 Doctoral Program in Biotechnology, University of Santiago, Santiago, Chile; 3 D. Alkalay L. Biotechnology Center, Federico Santa María University, Valparaíso, Chile *Corresponding author: pbarba@gmail.com Grey mold, caused by Botrytis cinerea, is a main grapevine disease in Chile. B. cinerea is a necrotrophic filamentous fungus of a complex agricultural management because of a broad host spectrum and to the different sources of inoculation. In the “Genome I: Botrytis-Grape Interaction” project, a macroarray formed by 4803 ESTs was designed. Gene expression studies using this macroarray allowed the comparison between mRNAs from infected and non-infected grapevine field plants from two contrasting cultivars: Thompson Seedless and Carménère. By this analysis, we established the involvement of a putative ankyrin-like protein highly induced in response to the pathogen infection. Although is not expected to find natural resistance sources to Botrytis in breeding grapevine studies using susceptible cultivars, the knowledge of highly responsible sequences to fungal challenge could be of significant relevance from a biotechnological approach. In this way, we propose that the promoter region of this gene may contain important responsive element which may provide information about the specificity of the plant response after fungal attack. A first step in this work was to verify and compare the observed induction of the grapevine ankyrin-like gene under fungal attack by specific real time PCR analysis of infected Carménère greenhouse plants. Afterwards, in silico analysis was carried out leading to the definition of an 890 base pair up-stream zone, which was experimentally characterized by use of GFP fusion constructs in tobacco transformation experiments. Wounding and Botrytis challenges were applied on stable transgenic lines, depicting differentially induced levels of gfp transcripts depending on the elicitor. Comparison of these heterologous inductions is under development by generation of the corresponding ´Thompson Seedless´ transgenic lines. 190 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
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O-40 Quantitative analysis of textu
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O-42 Transcriptional changes in res
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O-44 The Turkish grape (Vitis vinif
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Association genetics in relation wi
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O-47 Intravarietal variability of C
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O-49 Molecular survey of Georgian t
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O-51 Marker-assisted breeding: Iden
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O-53 An Integrated approach to stud
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O-55 Characteristics of promising m
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P-2 PMEI and SPY: Two genes with po
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P-4 Gene-assisted selection for tab
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P-6 Polyphenolic potential of the r
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P-8 Monoterpene levels in different
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P-10 Usefulness of the SCC8 scar ma
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P-12 The onset of berry ripening is
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P-14 Morphological characteristics
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P-16 A genetic analysis of berry qu
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P-18 Use of molecular markers for s
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P-20 Characterization of key compon
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P-22 Expression of early light-indu
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P-24 The development of molecular m
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P-26 Identification of ABA inducibl
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P-28 In vitro selection of HYP tole
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P-30 In silico SNP detection for ge
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P-32 Vitis vinifera genome annotati
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P-34 Genome-wide identification of
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P-36 Looking for genetic polymorphi
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P-38 Characterization of a new horm
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P-40 Towards a characterization of
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the overall identified polymorphism
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P-43 Proteomic characterization of
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P-45 Preliminary observations on th
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grapevine which will eventually be
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P-48 ‘Cioutat’: a somatic varia
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P-50 Estimation of protein spot var
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glucosyltransferase, anthocyanidin
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P-53 Marker-free transgenic grapevi
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P-55 Agrobacterium rhizogenes-media
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P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
Page 159 and 160: P-79 Studies on an excellent, early
Page 161 and 162: P-81 Effectiveness of different cul
Page 163 and 164: P-83 Some early maturing table grap
Page 165 and 166: P-85 New triploid seedless table gr
Page 167 and 168: P-87 Grape breeding and viticulture
Page 169 and 170: P-89 The usefulness of allotetraplo
Page 171 and 172: P-91 Drying rate of fresh berries f
Page 173 and 174: P-93 Development of table and raisi
Page 175 and 176: P-95 Reaction of grape rootstocks t
Page 177 and 178: P-97 Genetic analysis of the resist
Page 179 and 180: P-99 Berry cracking caused powdery
Page 181 and 182: P-101 Elicitation of grapevine defe
Page 183 and 184: P-103 The biological characteristic
Page 185 and 186: P-105 Pathogen responsiveness and v
Page 187: P-107 Screening grape hybrid famili
Page 191 and 192: P-112 Evaluation of grapevine germp
Page 193 and 194: P-114 Evaluation of four new rootst
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