conference schedule and program with abstracts - Horticulture ...
conference schedule and program with abstracts - Horticulture ...
conference schedule and program with abstracts - Horticulture ...
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
P-66<br />
Analysis of grape rootstocks by microsatellite markers<br />
G. Jahnke* 1 , G. Kocsisne Molnar 2 , J. Majer 1 , B. Szoke 1 , E. Tarczal 2 , P. Varga 1 , L. Kocsis 2<br />
1 Research Institute for Viticulture <strong>and</strong> Oenology, Centre of Agricultural Sciences, University of<br />
Pannonia, Badacsony, Hungary; 2 Department of <strong>Horticulture</strong>, University of Pannonia, Georgikon<br />
Faculty, Keszthely, Hungary<br />
*Corresponding author: gjahnke@mail.iif.hu<br />
The aim of this work was to determine the SSR profile of 96 Vitis accessions (mainly rootstocks)<br />
at 19 loci <strong>and</strong> to find genetic relatedness between them. Molecular markers used in the last 30<br />
years are not effected by the environment, giving a good tool for determination of genetic<br />
distances. Based on our former experiences SSR analysis was carried out on 96 grape accessions,<br />
mainly rootstocks. DNA was extracted from young leaves. Analyses were carried out using 19<br />
microsatellite loci. The loci were selected to cover all of the linkage groups to get more exact<br />
impression of the genome of the grape rootstocks. Results of SSR analysis <strong>and</strong> a dendogram<br />
showing genetic relatedness were constructed. Based on the results it can be established that<br />
most of the loci showed appropriate polymorphism. High similarity was detected between the<br />
clones of T5C <strong>and</strong> TK5BB. Strong differences were detected between the so called “clones” of<br />
T5C <strong>and</strong> TK5BB, suggesting, that they are not real clones, but different genotypes <strong>with</strong> highly<br />
similar morphological features. The Vitis sylvestris accessions showed close relatedness <strong>with</strong> the<br />
Vitis vinifera L. varieties.<br />
146